NGHIÊN CỨU ẢNH HƯỞNG CỦA CƯỜNG ĐỘ TIA GAMMA NGUỒN COBALT 60 LÊN TỶ LỆ SỐNG VÀ SINH TRƯỞNG CỦA CÂY DẠ YẾN THẢO IN VITRO (Petunia hybrida)

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Tác giả: Thị Thúy Lê, Thị Ngọc Mai Vũ

Ngôn ngữ: vie

Ký hiệu phân loại:

Thông tin xuất bản: Tạp chí Khoa học Công nghệ và Thực phẩm (Tên mới: Tạp chí Khoa học Đại học Công Thương), 2020

Mô tả vật lý: tr.49

Bộ sưu tập: Metadata

ID: 329606

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Nghiên cứu được thực hiện để đánh giá tác động của cường độ tia gamma 60Co đến khả năng sống, tạo chồi và sinh trưởng từ 2 vật liệu chiếu xạ khác nhau của cây dạ yến thảo trong điều kiện nuôi cấy in vitro, từ đó làm cơ sở để đánh giá các biến dị sau này. Trong thí nghiệm, mẫu mô sẹo in vitro và các chồi cây dạ yến thảo in vitro, giống hoa đơn màu hồng và nhị màu trắng được chiếu xạ ở các liều lượng khác nhau từ 0 Gy đến 80 Gy. Kết quả cho thấy, đối với mẫu mô sẹo in vitro, liều chiếu xạ càng cao thì tỷ lệ sống, tạo chồi và sinh trưởng của chồi càng giảm. Ở nghiệm thức chiếu xạ 40 Gy, một số chồi có sự khác biệt về hình thái rõ ràng so với các cường độ chiếu xạ khác, ở cường độ chiếu xạ 60 Gy và 80 Gy mẫu chết hoàn toàn. Đối với mẫu lá từ cây dạ yến thảo in vitro chiếu xạ, liều chiếu xạ càng cao tỷ lệ sống và tạo chồi giảm, tuy nhiên các chỉ tiêu sinh trưởng được theo dõi lại tăng. Tỷ lệ sống ở cường độ chiếu xạ 60 Gy là 55,56%, chồi ở nghiệm thức này có số lá hình thành nhiều, chiều dài lá và chiều cao chồi đạt giá trị cao nhất, hình dạng khác biệt rõ ràng so với các chồi ở nghiệm thức khác. Mẫu cấy chết hoàn toàn ở cường độ chiếu xạ 80 Gy.This study aimed to determine the effect of gamma radiation on the survival sample rate, shoot generation, and the growth of two different irradiated materials of petunia in vitro, as a basis to create many materials for breeding. In the experiment, in vitro callus samples and petunia shoots of pink single - flower and white stamens petunia variety were irradiated by different doses of gamma-ray from 0 Gy to 80 Gy. The results showed that, for in vitro callus samples, the higher the radiation dose, the lower the growth of shoots decreased. In the 40 Gy irradiation dose, some of the shoots had a distinct morphological difference from the other irradiation intensities. The samples died completely at 60 and 80 Gy. For leaf samples from irradiated in vitro petunia plant, the higher the irradiated dose, the higher the survival and shoot formation rate decreased, but the observed growth criteria increased. The survival rate at 60 Gy irradiation intensity was 55.56%, the shoots had the highest number of leaves formation, leaf length, and height of shoots, and their shapes were different from the others. The sample died completely at an irradiation intensity of 80 Gy.

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