Chiết trắc quang xác định nimodipin bằng sự hình thành phức cặp ion với bromthymol xanh

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Tác giả: Thị Tuệ Minh Hoàng, Thị Hoa Lê, Thu Hà Nguyễn, Trung Dũng Nguyễn

Ngôn ngữ: vie

Ký hiệu phân loại:

Thông tin xuất bản: Journal of Science and Technique, 2022

Mô tả vật lý: tr.5

Bộ sưu tập: Metadata

ID: 329633

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A new spectrophotometric method was established for the determination of nimodipine (NMD) in pharmaceutical formulations. This method is based on the formation of a yellow ion-pair complex between the reduced nimodipine and bromothymol blue (BTB) in an acidic medium. Chloroform was used as an optimal solvent for the extraction of ion-pair complex and measured the absorbance of this complex at wavelengths of 414.5 nm. The effect of optimum condition via extracting solvent, volume of dye, shaking time, reaction ratio was studied. Beer’s law is obeyed in the concentration ranges of 4.0-50.0 μg/mL with a molar absorptivity of 1.50x104 L.mol-1.cm-1 and limit of detection (LOD) of 1.87 μg/mL. The accuracy and precision of the method were evaluated on an intra-day and inter-day basis. The relative error (%RE) and the relative standard deviation (%RSD) were <
5.0% while the recovery (%) was 99.05-103.25. The proposed method was applied successfully to determine nimodipine in pharmaceutical preparation (Nomitop, Bayer).Phương pháp quang phổ mới đã được thiết lập để xác định nimodipin trong các chế phẩm dược. Phương pháp này dựa trên sự hình thành phức cặp ion màu vàng giữa dạng khử của nimodipin và thuốc thử bromothymol xanh (BTB) trong môi trường axit. Chloroform được sử dụng như một dung môi tối ưu để chiết phức cặp ion và độ hấp thụ quang được đo ở bước sóng 414,5 nm. Ảnh hưởng của một số thông số như dung môi chiết, thể tích thuốc nhuộm, thời gian lắc chiết, tỉ lệ phản ứng đã được nghiên cứu. Khoảng nồng độ nimodipin tuân theo định luật Bia là 4,0-50,0 μg/mL, với độ hấp thụ mol của phức là 1,50×104 L.mol-1.cm-1 và giới hạn phát hiện (LOD) là 1,87 μg/mL. Độ chính xác và độ chụm của phương pháp được đánh giá trên cơ sở nghiên cứu trong và giữa các ngày, phần trăm sai số tương đối (%RE) và độ lệch chuẩn tương đối (%RSD) bé hơn 5,0%, độ thu hồi (%) là 99,05-103,25. Phương pháp đề xuất đã được áp dụng thành công để xác định nimodipin trong chế phẩm dược Nomitop (Bayer, Đức).

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