The glassy carbon electrode electrodeposited platinum nanoflowers on the surface to form a new electrode (PtNFs/GCE).The behavior of PtNFs/GCE has been studied for Amoxicillin (AMX) determination by square wave anodic stripping voltammetry (SWASV). After optimizing the experimental and voltammetric conditions, the best stripping peak obtained current intensity in 0.2 M phosphate buffer solution (pH = 7.5) with 150 s accumulation time, 60 mV pulse amplitude, and 0.25 V.s-1 scan rate. The limit of detection and quantity was observed as 0.113 µM and 0.372 µM, respectively. Under suitable conditions, the method gained high reproducibility: RSD = 9.65% (n=5). Interference effects of some β-lactam antibiotics and other possible interferent were studied. Finally, the PtNFs/GCE was successfully applied to analyze Amoxicillin antibiotic residues in aquaculture wastewater, resulting in satisfactory statistical agreement with high-performance liquid chromatography (HPLC).