Kết quả nghiên cứu điển hình và kiểu gen kháng kháng sinh của vi khuẩn Escherichia Coli sản sinh ESBL phân lập từ chất thải lợn

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Tác giả: Anders Dalsgaard, Thị Thanh Sơn Đặng, John Elmerdahl Olsen, Mahuton Gildas Hounmanou, Thị Nhật Trần, Thị Hương Giang Trương, Thị Quý Dương Trương

Ngôn ngữ: vie

Ký hiệu phân loại:

Thông tin xuất bản: Tạp chí Khoa học kỹ thuật Thú y, 2020

Mô tả vật lý: tr.30

Bộ sưu tập: Metadata

ID: 330518

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Antimicrobial resistance bacteria is a threat to public health in the worldwide and transmission of the resistant genes is the most important antibiotic resistance mechanism of bacteria. A total of 43 E. coli strains producing ESBL isolated from the pig feces samples in Bac Ninh province were used for analyzing their gene sequences by Illumina Nextera XT method using v3 protocol of MiSeq kit. The studied result showed that the antibiotic resistance genes identified from the above isolated bacteria strains included blaCTX-M-55(22/43), blaCTX-M-14 (10/43), blaCTX-M-27 (5/43), blaCTX-M-15 (2/43) and blaOXA10 (4/43). There was 1 isolate producing ESBL carried both blaDHA, AmpC genes (the gene resisted beta-lactam antibiotics, C group). There were 18/43 E. coli isolates carried the plasmid-mediated mcr-1 gene (resisted colistin). Of which, there were 4 isolates carried the mcr-3 gene (resisted colistin), 2 isolates had mutations in the prmBgene (p.V161G). Most of the E. coli strains (41/43) producing ESBL harbored plasmid-mediated genes resisted beta-lactam antibiotics (quinolone group) (qnrS1 and/or qnrB6 and/or qnrB19) and/or quinolone resistance due to mutations. These resistant genes could be transmitted following the horizontal or vertical ways among different bacterial strains/species. This is one of the witness on spreading the antibiotic resistance genes of E. coli strains isolated from the pig waste in Viet NamVi khuẩn kháng kháng sinh là mối đe dọa đối với sức khỏe cộng đồng trên toàn thế giới và lây truyền gen kháng là cơ chế kháng kháng sinh quan trọng nhất của vi khuẩn. Trong nghiên cứu này, 43 chủng E. coli sản sinh ESBL phân lập từ chất thải của lợn tại Bắc Ninh đã được lựa chọn để giải trình tự gen của chúng bằng phương pháp Illumina Nextera XT sử dụng quy trình v3 của kit thử MiSeq. Kết quả nghiên cứu cho thấy gen kháng kháng sinh phát hiện được từ các chủng vi khuẩn nói trên bao gồm: blaCTX-M-55 (22/43), blaCTX-M-14(10/43), blaCTX-M-27 (5/43), blaCTX-M-15 (2/43) và blaOXA10 (4/43). Một chủng E. coli sinh ESBL mang gen blaDHA - gen quy định tính kháng kháng sinh beta-lactam nhóm C. 18/43 chủng E. coli sản sinh ESBLmang gen mcr-1 (kháng colistin). Trong đó có 3 chủng đồng mang gen kháng colistin mcr-3, 2 chủng có đột biến trên gen prmB (p.V161G). 41/43 (95,3%) chủng E. coli sản sinh ESBL đều mang gen kháng nhóm kháng sinh quinolone (qnrS1 hoặc/và các gen kháng qnrB6, qnrB19) qua plasmid (PMQR). Các gen kháng này có thể truyền ngang hoặc truyền dọc giữa các chủng/ loài vi khuẩn khác nhau và đây là một trong những bằng chứng về lây lan gen kháng kháng sinh của vi khuẩn E. coli phân lập từ chất thải của lợn tại Việt Nam

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