Phân tích trình tự một phần gen nucleoprotein (N) của chủng virus dại tại Hà Tiên, Kiên Giang

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Tác giả: Đức Hiền Nguyễn, Phúc Vinh Trương

Ngôn ngữ: vie

Ký hiệu phân loại:

Thông tin xuất bản: Tạp chí Khoa học kỹ thuật Thú y, 2020

Mô tả vật lý: tr.45

Bộ sưu tập: Metadata

ID: 331033

Thêm vào giỏ Liên kết toàn văn

Nghiên cứu này được thực hiện với mục đích xác định đặc điểm của virus dại (chi Lyssavirus,họ Rhabdoviridae, bộ Mononegavirales) bằng cách giải trình tự một phần gen nucleoprotein (N) đểbiết kiểu gen và phát sinh loài của chủng virus dại 1230.2019 phát hiện trên một chó mắc bệnh dại ởHà Tiên, tỉnh Kiên Giang, Việt Nam. Mẫu não được thu, vô trùng từ chó chết để phát hiện virus dại.Mẫu này đã được phát hiện dương tính bằng phương pháp RT-PCR và giải trình tự cho nồng độ sảnphẩm gen tốt.Trình tự nucleotide và trình tự amino acid của chủng vi khuẩn dại 1230.2019 với các chủng virusdại tham chiếu có mức độ giống nhau lần lượt là 95,20–100% và 92,00–100%. Chủng virus dại1230.2019 ở chó bệnh Hà Tiên (Việt Nam) cùng quan hệ di truyền với 8 chủng virus dại tham chiếutrong nghiên cứu. Chủng 1230.2019 có kết quả phân tích khoảng cách di truyền (0-0,16%) rất gầnvới 5 chủng virus dại vacxin tham chiếu và ba chủng virus dại ở chó bệnh ở GenBank. Phả hệ dựavào chuỗi gen nucleoprotein (N), chủng 1230.2019 có mức độ đồng nhất gần như tuyệt đối và tươngđồng cao với chủng vacxin Pháp 93127FRA (vacxin RABISIN ngừa dại đang được sử dụng rộng rãiở Việt Nam).This study was performed with the aim of characterization of the rabies virus (genus: Lyssavirus of the family Rhabdoviridae under the order Mononegavirales) by sequencing partial nucleoprotein (N) gene of the rabies virus and phylogenetic analysis to know genotype and genealogy of rabies virus 1230.2019 in the infected dogs in Ha Tien, Kien Giang province, Viet Nam. A brain sample was aseptically collected from a dead dog to detect rabies virus. This sample was found to be positive through RT-PCR assay, with high content genetic products through sequencing.Arrangement of the nucleotide and amino acid sequences of the rabies strain 1230.2019 with reference rabies strains showed the similarity of 95.20–100% and 92.00–100%, respectively. Rabies virus strain 1230.2019 of infected dog in Ha Tien (Viet Nam) has the same genetic relationship with eight reference rabies virus in the study. Strain 1230.2019 has genetic distance analysis results (0-0.16%) very close to five reference rabies vaccine strains and three strains of rabies virus in infected dogs in GenBank. Genealogy based on nucleoprotein (N) gene sequence, strain 1230.2019 has almost absolute homogeneity and high similarity with French vaccine strain 93127FRA (RABISIN vaccine against rabies is widely used in Viet Nam)

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