Đánh giá sự phát triển của trứng giun đũa cho Toxocara Canis trong các môi trường Formol 1%, Formol 2%, Formol 4% và acid sunfuric 0,1N

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Tác giả: Thế Anh Đồng, Thị Ngần Lưu, Thị Hoa Mạc, Thị Hoàng Yến Nguyễn, Thị Lan Anh Nguyễn, Thị Quỳnh Nguyễn

Ngôn ngữ: vie

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Thông tin xuất bản: Tạp chí Khoa học kỹ thuật Thú y, 2020

Mô tả vật lý: tr.67

Bộ sưu tập: Metadata

ID: 331038

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Nghiên cứu được tiến hành nhằm đánh giá sự phát triển của trứng Toxocara canis trong các môitrường formol 1%, formol 2%, formol 4% và H2SO4 0,1N
từ đó lựa chọn được môi trường thích hợpnhất cho sự phát triển của trứng T. canis. (T. canis là một trong những tác nhân gây toxocariasis ởngười). Chẩn đoán toxocariasis trên người do Toxocara spp. chủ yếu dựa vào kỹ thuật ELISA sử dụngkháng nguyên chất tiết từ ấu trùng gây nhiễm (ATGN) của T. canis. Quy trình sản xuất kháng nguyênnày được thực hiện qua nhiều bước, trong đó bước nuôi trứng T. canis đến giai đoạn chứaATGN là quantrọng nhất. Trong nghiên cứu này chúng tôi nuôi trứng T. canis thu từ tử cung của giun trưởng thành ởgiai đoạn một tế bào đến giai đoạn chứa ATGN trong các môi trường khác nhau. Kết quả thu được nhưsau: Tỷ lệ phát triển của trứng T. canis đến giai đoạn chứa ATGN cao nhất khi nuôi trong môi trườngH2SO4 0,1N (83,31%). Bên cạnh đó, tỷ lệ phát triển của trứng T. canis đến ATGN trong formol 1%,formol 2% và formol 4% lần lượt là 73,33%
71, 05% và 68,61%. Như vậy, kết quả nghiên cứu chỉ rarằng môi trường H2SO4 0,1N là môi trường thích hợp nhất cho sự phát triển của trứng T. canis.The objective of this study aimed at evaluating the development of Toxocara canis eggs in formol 1%, formol 2%, formol 4% and H2SO4 0.1N media, then to choose the best culture medium for the development of T. canis eggs (T. canis is one of the agents causing toxocariasis in humans). The diagnosis of this infection in humans due to Toxocara spp. was mainly based on ELISA technique using secretory/excretory (ES) antigen from the infective T. canis larvae. The procedure of making ES antigen consisted of some steps, of which, the step of culturing T. caniseggs to larvae containing secretory/excretory (ES) antigen was the most important. In this study we cultured T. canis eggs collecting from uterus of adult worms in different media. The studied result showed that the rate of the development from T. canis eggs to larvae stage containing secretory/excretory (ES) antigen in H2SO4 0.1 N medium was 83.31%, and this was the medium showing the best development and the lowest extraordinary rate of T. canis eggs. Besides, the rates of the development of T. canis egg to the above larvae stage in formol 1%, formol 2%, formol 4% media were 73.33%, 71.05% and 68.61%, respectively. In conclusion, the result of this study showed that H2SO4 0.1N medium is the best medium for the development of T. canis eggs.

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