Virus dịch tả heo Châu Phi trên thai heo sảy và kết quả âm tính giả của REALTIME- PCR

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Tác giả: Ngọc Hải Nguyễn, Thị Kim Yến Nguyễn, Thị Ngọc Nguyễn, Thị Phương Bình Nguyễn, Trung Quân Nguyễn, Hoàng Anh Thư Trần

Ngôn ngữ: vie

Ký hiệu phân loại:

Thông tin xuất bản: Tạp chí Khoa học kỹ thuật Thú y, 2020

Mô tả vật lý: tr.5

Bộ sưu tập: Metadata

ID: 331077

Thêm vào giỏ Liên kết toàn văn

African swine fever (ASF) has appeared in Viet Nam since February, 2019 and caused the huge economic losses for the sector of pig husbandry. Up to now, the ASF epidemic has temporarily passed, but ASF virus is still remaining in the pig herds, especially in the sow farms where the ASF epidemic has happened. The sows did not show any specific clinic signs until miscarriage/absortion. The result of RT-PCR tests for PRRS virus, classical swine fever virus, and PCR tests for Parvovirus and Aujeszky’s virus on the foetus of abortion were all negative. However, the result of testing ASF virus on 5 abortus samples from the different sows by conventional PCR procedure (OIE) in the study were positive, while the result of parallel test for ASF virus according to the realtime-PCR (OIE) procedure was negative. The research results allow us to confirm that ASF virus is circulating in the sows in Viet Nam, and the false negative results of realtime-PCR test by (OIE) procedure for ASF virus in the foetus of abortion samples were recorded.Bệnh dịch tả heo châu Phi xuất hiện ở Việt Nam từ tháng 2/2019 và đã gây thiệt hại kinh tế rất lớn cho ngành chăn nuôi heo. Dịch bệnh tạm thời đã qua đi, nhưng trên thực tế virus DTHCP vẫn còn tồn tại trên đàn heo nuôi, nhất là đàn heo sinh sản tại các trại đã từng bị mắc bệnh DTHCP. Heo nái không có biểu hiện lâm sàng bất thường cho đến khi bị sảy thai. Các xét nghiệm RT-PCR đối với virus PRRS, dịch tả heo cổ điển, và PCR đối với Parvovirus và Aujeszky's trên thai sảy đều cho kết quả âm tính. Tuy nhiên, xét nghiệm virus DTHCP trên 5 mẫu thai sảy từ các heo nái khác nhau bằng quy trình PCR thông thường (OIE) trong nghiên cứu này đều cho kết quả dương tính, trong khi xét nghiệm song song theo quy trình realtime-PCR (OIE) cho kết quả âm tính. Kết quả nghiên cứu đã cho phép khẳng định virus DTHCP đang lưu hành trên đàn heo nái ở Việt Nam, đồng thời ghi nhận kết quả âm tính giả của quy trình realtime-PCR (OIE) trong xét nghiệm virus DTHCP trên thai sảy

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