Tính an toàn và miễn dịch của virus dịch tả heo Châu Phi nhược độc, chủng G-Delta- I177L trên heo

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Tác giả: Anh Thy Bùi, Huỳnh Thiên Thanh Đào, Thanh Thủy Đỗ, Nguyễn Hải Vy Hồ, Thị Ngọc Ánh Huỳnh, Thị Thu Phương Lê, Đức Huy Nguyễn, Quang Huy Nguyễn, Tấn Liêm Nguyễn, Thanh Hoà Nguyễn, Văn Dung Nguyễn, Thị Yến Như Phạm, Vô Ngôn Quách, Hữu Huy Trần, Xuân Hạnh Trần

Ngôn ngữ: vie

Ký hiệu phân loại:

Thông tin xuất bản: Tạp chí Khoa học kỹ thuật Thú y, 2020

Mô tả vật lý: tr.14

Bộ sưu tập: Metadata

ID: 331114

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The study was carried out with the aims to test safety and immunity of the attenuated ASFV strain G-delta-I177L. The studied results showed that ASFV strain G-delta-I177L belonged to genotype II, possessed ability to multiply well on the PBMC and BMC cells.ASFV strain G-delta-I177L was safe to inject to pigs even when using high dose of 106 HAD50, 104 times higher than that of minimum immunizing dose (102 HAD50) to protect 100% pigs. By challenge experiments demonstrated that the pigs that injected with ASFV strain G-deltaI177L could stimulate immune response and be able to protect pigs against the virulent ASFV causing ASF in pigs in Viet Nam. This proves that ASFV strain G-delta-I177L has matching protective antigens to the virulent ASFV circulating in Viet Nam. From the studied results obtained, it is suggested that ASFV strain G-delta-I177L can be used to product ASF vaccine against ASF for pigs raising in Viet NamThí nghiệm được thực hiện nhằm đánh giá tính an toàn và miễn dịch của chủng virus DTHCP nhược độc, chủng G-delta-I177L. Kết quả nghiên cứu cho thấy virus chủng G-delta-I177L thuộc genotype II, có khả năng nhân lên tốt trên tế bào PBMC và BMC. Chủng virus này an toàn khi tiêm cho heo, ngay cả khi sử dụng liều cao 106HAD50, gấp 10.000 lần liều miễn dịch bảo hộ 100% heo thí nghiệm.Bằng phương pháp thử thách cường độc, heo tiêm virus DTHCP chủng G-delta-I177L kích thích đáp ứng miễn dịch tốt và có khả năng bảo hộ được heo chống lại virus DTHCP cường độc gây bệnh DTHCP cho heo tại Việt Nam. Điều này chứng tỏ virus DTHCP nhược độc chủng G-delta-I177L có tính tương đồng kháng nguyên bảo hộ với virus DTHCP cường độc lưu hành tại Việt Nam. Từ kết quả nghiên cứu thu được, có thể dùng chủng virus DTHCP G-delta-I177L để sản xuất vacxin phòng bệnh DTHCP cho đàn heo nuôi tại Việt Nam.

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