Xác định tiềm năng ức chế virus dịch tả lợn Châu Phi của một số hoạt chất thảo dược

0 Người đánh giá. Xếp hạng trung bình 0

Tác giả: Thị Tố Nga Bùi, Trần Anh Đào Bùi, Hữu Anh Đặng, Minh Sơn Hoàng, Thị Phương Hoàng, Văn Trường Lê, Thị Giang Nguyễn, Thị Hoa Nguyễn, Thị Lan Nguyễn, Thị Thanh Hà Nguyễn, Minh Hải Trần, Phúc Thanh Sang Vũ, Thị Thu Trà Vũ

Ngôn ngữ: vie

Ký hiệu phân loại:

Thông tin xuất bản: Tạp chí Khoa học kỹ thuật Thú y, 2020

Mô tả vật lý: tr.25

Bộ sưu tập: Metadata

ID: 331160

Thêm vào giỏ Liên kết toàn văn

Nghiên cứu được thực hiện nhằm đánh giá khả năng ức chế virus dịch tả lợn châu Phi (DTLCP)của 9 đơn chất có nguồn gốc thảo dược gồm: glycyrrhizin, berberine, emodin, ellagic acid, methylgallate, caffeic acid, kaempferol, baicalein và naringenin. Thử nghiệm khả năng gây độc tế bào trênđại thực bào phế nang lợn cho thấy glycyrrhizin, berberine, ellagic acid, methyl gallate, kaempferolvà naringenin không gây độc tế bào, emodin, caffeic acid và baicalein gây độc tế bào ở nồng độ 200µM/ml. Kết quả thử nghiệm khả năng ức chế virus DTLCP của các đơn chất cho thấy berberine vàemodin thể hiện khả năng ức chế virus DTLCP rõ rệt. Sau 48 giờ ủ berberine (nồng độ 200 µM/ml)với virus DTLCP, hiệu giá của virus giảm từ 107,47 xuống 105,80
còn với emodin ở nồng độ 150 µM/ml thì hiệu giá virus giảm từ 107,47 xuống còn 106,47The study was carried out to evaluate the inhibitory ability of 9 herbal ingredients including: glycyrrhizin, berberine, emodin, ellagic acid, methyl gallate, caffeic acid, kaempferol, baicalein and naringenin to African swine fever virus (ASFV). The test for cytotoxicity on porcine alveolar macrophages by these herbal ingredients showed that glycyrrhizin, berberine, ellagic acid, methyl gallate, kaempferol and naringenin did not cause cytotoxic, emodin, caffeic acid and baicalein caued cytotoxic at a concentration of 200 µM/ml. The results of testing the ability to inhibit ASFV of the single substance showed that berberine and emodin showed a significant ability to inhibit ASFV. After 48 hours of incubating 200 µM/ml of berberine with ASFV, the viral titer decreased from 107.47 to 105.80
and with emodin at a concentration of 150 µM/ml, the viral titer decreased from 107.47 to 106.47.

1. Đọc trực tuyến

1. Đọc trực tuyến

Tạo bộ sưu tập với mã QR