Phân bố các serotype và gen độc tố của vi khuẩn Glaesserella parasuis phân lập từ Việt Nam và Trung Quốc

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Tác giả: Thị Hoa Nguyễn, Văn Chào Nguyễn, Thị Thanh Tâm Vũ

Ngôn ngữ: vie

Ký hiệu phân loại:

Thông tin xuất bản: Tạp chí Khoa học kỹ thuật Thú y, 2020

Mô tả vật lý: tr.59

Bộ sưu tập: Metadata

ID: 331169

Thêm vào giỏ Liên kết toàn văn

Glaesserella parasuis (G. parasuis) trước đây có tên là Haemophilus parasuis là vi khuẩn thường trú ởđường hô hấp của lợn, vi khuẩn này là nguyên nhân gây bệnh Glasser với các đặc điểm viêm khớp, viêmmàng giả và viêm màng não. Bệnh Glasser là một trong những nguyên nhân chính làm tăng tỷ lệ chết ởlợn con, làm giảm hiệu quả kinh tế trong chăn nuôi lợn. Trong nghiên cứu này, các serotype và 9 gen độctố (VG) của 400 chủng G. parasuis phân lập từ mẫu thực địa đã được lần lượt phân tích bằng phương phápone-step multiplex PCR (mPCR) và phương pháp PCR. Tương quan giữa serotype và gen độc tố cũng đãđược phân tích. Kết quả cho thấy, các serotype phổ biến ở tất cả các chủng là serotype 5/12, 4, 1 và 6. Cósự khác nhau về sự phân bố các serotype 1, 2 và 10 giữa chủng phân lập ở Việt Nam và ở Trung Quốc (p<
0,05). Đa số các chủng G. parasuis mang ít nhất 1 trong 9 gen độc tố của vi khuẩn này, trong khi đó chỉcó 16/400 chủng không mang VG. Trong đó tỷ lệ các chủng mang gen vta1 (91,3%) là cao nhất, tiếp đếnlà các gen vta3 (86,5%), vta2 (82,5%), và wza (68,0%). Một số mối tương quan thuận giữa serotype vàVG cũng đã được phát hiện, trong đó có tương quan giữa serotype 1 với các gen wza, HPM-1373, vta2,vta3
giữa serotype 5 với các gen lgsB, capD, HPM-1371, HPM-1372
giữa serotype 6 với gen vta2
vàgiữa serotype 7 với các gen wza, HPM-1371, HPM-1372. Sự phân bố các gen độc tố ở chủng có độc lựccao cũng cao hơn so với các chủng có độc lực trung bình và độc lực thấp. Kết quả của đề tài này cho thấysự đa dạng của các serotype và các VG của các chủng G. parasuis phân lập được. Hơn nữa, nghiên cứucũng cung cấp những thông tin quan trọng, làm cơ sở cho việc lựa chọn hoặc phát triển các loại vacxinphù hợp để kiểm soát bệnh Glasser ở Việt Nam và Trung QuốcGlaesserella parasuis resently still known as Haemophilus parasuis is a common opportunistic pathogen known for its ability to colonize in the healthy pigs and causes Glasser’s disease with the characteritics of fibrinous polyserositis, arthritis and meningitis. Glasser’s disease is one of the major causes of high mortality rates in piglets, resulting in significant economic losses to the swine production in the worldwide. In this study, the serotypes and 9 known virulence-associated genes (VGs) of 400 G. parasuis field strains were identified by one-step multiplex PCR (mPCR), and PCR method, respectively. The correlations between serotypes and VGs were analysed. The studied results showed that the most prevalent serotypes were 5/12, 4, 1, and 6 for all the strains. However, difference was observed between Chinese isolates and Vietnamese isolates in which the dominant serotypes were 5/12, 4, and 1 for the Chinese strains and 5/12, 2 and 4 for the Vietnamese ones. The prevalence of serotypes 1, 2, 10, and non-typeable were significantly different between the Chinese and Vietnamese strains (p<
0.05). Most of strains carried at least one of the nine tested VGs, while only 16 strains did not carry any VG. The vta1 (91.3%) was the most frequently detected, followed by vta3 (86.5%), vta2(82.5%), and wza (68.0%). Several positive associations between serotypes and VGs were observed which were between serotype 1 and wza, HPM-1373, vta2, and vta3
serotype 5 and lgsB, capD, HPM-1371, and HPM-1372
serotype 6 and vta2
and serotype 7 and wza, HPM-1371, and HPM-1372. The prevalence of VGs was significantly higher among strains belonging to the highly virulent group than those belonging to the moderate and non-virulent groups. The results in this study confirmed the diversity and prevalence of serotypes and VGs in G. parasuis field strains. This is important for developing vaccine candidates allowing for the control of Glasser’s disease in China and Viet Nam.

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