Bacillus velezensis strain BL-1 was successfully isolated from the soil samples collected from Quang Xuong district, Thanh Hoa province, Vietnam. The BL-1 strain was identified as Bacillus velezensis based on morphological and phylogenetic analysis of its 16S rRNA gene. Our study proposed a protocol for mass production of B. velezensis BL-1 using a soybean flour molasses-based medium which provided a high efficiency. The optimized fermentation conditions were an incubation for 48 hours at 35oC with a shaker at speed of 120 rpm, pH 7.0, and inoculum volume of 2% (v/v).