NGHIÊN CỨU HOẠT TÍNH KHÁNG OXY HÓA VÀ KHÁNG KHUẨN CỦA CHIẾT XUẤT ETHANOL TỪ TẢO LỤC (Caulerpa racemosa) TẠI TỈNH KIÊN GIANG

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Tác giả: Hoàng Phúc Nguyễn, Thị Kim Huê Nguyễn, Thanh Mến Trần

Ngôn ngữ: vie

Ký hiệu phân loại:

Thông tin xuất bản: Tạp chí Khoa học và Công nghệ - Đại học Thái Nguyên, 2022

Mô tả vật lý: tr.83-91

Bộ sưu tập: Metadata

ID: 332075

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Chiết xuất ethanol từ tảo lục Caulerpa racemosa được thu thập tại Hòn Sơn, tỉnh Kiên Giang bước đầu được khảo sát thành phần hóa học, đánh giá hoạt tính kháng oxy hoá và kháng khuẩn. Hoạt tính kháng oxy hóa của chiết xuất được xác định bằng phương pháp DPPH (2,2-Diphenyl-1-picrylhydrazyl), ABTS (2,2'-Azinobis 3-ethylbenzthiazoline-6-sulfonic acid), phosphomolypdenum và khử sắt RP (Reducing power). Kết quả khảo sát cho thấy, chiết xuất Caulerpa racemosa thể hiện hoạt tính kháng oxy hóa tốt nhất ở hai phương pháp ABTS và phosphomolypdenum với các giá trị EC50 lần lượt là 174±3,48 µg/mL, 206±1,07 µg/mL. Hoạt tính kháng vi khuẩn được khảo sát trên sáu chủng vi khuẩn gây bệnh trên người. Kết quả đã chứng minh chiết xuất có hiệu quả trong hoạt động kháng khuẩn trên bốn chủng vi khuẩn Bacillus subtilis, Bacillus cereus, Listeria innocua, và Staphylococcus aureus. Bên cạnh đó, nghiên cứu còn ghi nhận hàm lượng phenolic và flavonoid tổng số trong chiết xuất lần lượt là 56,7±2,34 mg GAE/g chiết xuất và 128±2,69 mg QE/g chiết xuất. Từ các kết quả nghiên cứu cho thấy, tảo lục Caulerpa racemosa có tiềm năng ứng dụng trong dược phẩm cũng như thực phẩm.The ethanol extract from the green algae Caulerpa racemosa collected at Hon Son island, Kien Giang province was initially investigated its chemical compositions, and evaluated its antioxidant and antibacterial activities. The antioxidant activity of the ethanolic extract C. racemosa was determined by methods of DPPH (2,2-Diphenyl-1-picrylhydrazyl), ABTS (2,2'-Azinobis 3-ethylbenzthiazoline-6-sulfonic acid), phosphomolybdenum and RP (Reducing power). The results indicated that ABTS and phosphomolybdenum method showed highest antioxidant activity with EC50 values of 174±3.48 µg/mL anh 206±1.07 µg/mL, respectively. The antimicrobial activity was investigated on six strains of human pathogenic bacteria. This result demonstrated that the extract was effective in antibacterial activity against four strains of Bacillus subtilis, Bacillus cereus, Listeria innocua, and Staphylococcus aureus. In addition to these biological activities, the study also examined phenolic and flavonoid total contents in the extract with amounts of 56.7±2.34 mg GAE/g and 128±2.69 mg QE/g, respectively. From these preliminarily experimental results, the extract of green algae Caulerpa racemosa has potential applications in production of pharmaceuticals and functional foods.

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