ẢNH HƯỞNG CỦA NƯỚC DỪA, AGAR VÀ SUCROSE ĐẾN THUỶ TINH HOÁ, SỰ TÁI SINH VÀ SỰ ỔN ĐỊNH DI TRUYỀN CỦA CÂY CẨM CHƯỚNG IN VITRO

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Tác giả: Việt Hồng La, Văn Đính Nguyễn

Ngôn ngữ: vie

Ký hiệu phân loại:

Thông tin xuất bản: Tạp chí Khoa học và Công nghệ - Đại học Thái Nguyên, 2022

Mô tả vật lý: tr.20-27

Bộ sưu tập: Metadata

ID: 332145

Thêm vào giỏ Liên kết toàn văn

Thuỷ tinh hoá là một vấn đề thường xảy ra, làm giảm chất lượng cây cẩm chướng (Dianthus caryophyllus L.) in vitro, dẫn tới giảm hiệu quả quy trình sản xuất. Trong nghiên cứu này, nước dừa, sucrose và agar được thăm dò hàm lượng bổ sung vào môi trường nhằm tái sinh chồi in vitro và làm giảm tỷ lệ thuỷ tinh hoá. Việc đánh giá sự ổn định di truyền của cây tái sinh cũng được thực hiện. Kết quả cho thấy, môi trường MS, agar 7 g.L-1, sucrose 30 g.L-1 bổ sung nước dừa 5% (v/v) là thích hợp để tái sinh các giống trắng viền đỏ, hồng cánh sen, đỏ nhung và đỏ chùm, số chồi/mẫu lần lượt là 5,75
6,00
4,25 và 5,00. Chiều cao chồi lần lượt là 6,25
6,38
8,00
8,50 (cm)
Số lá/chồi tương ứng là 11,75
12,50
12,00
11,75. Trong khi môi trường tương tự nhưng bổ sung nước dừa 10% (v/v) là phù hợp cho giống vàng chanh. Số chồi/mẫu, chiều cao chồi và số lá/chồi lần lượt là 5,75
8,50
11,25. Môi trường MS, agar 7 g.L-1 bổ sung sucrose 35g hoặc MS, sucrose 30 g.L-1 bổ sung agar 8 g.L-1 thích hợp để giảm tỷ lệ thuỷ tinh hoá ở năm giống cẩm chướng
trong khi đó, agar 7 g.L-1 hoặc sucrose 30 g.L-1 là phù hợp để tái sinh chồi in vitro. Bằng chỉ thị UBC 808, 5’-G-(AG)7-C-3’, chứng minh cây tái sinh ổn định di truyền như cây mẹ.Vitrification is a severe common problem, causing a reduction in the quality of in vitro carnation (Dianthus caryophyllus L.), leading to reduced process efficiency of production. In this study, coconut water, sucrose and agar were added to the medium to regenerate shoots in vitro and reduce the rate of vitrification. Evaluation of the genetic stability of regenerated plants was also performed. The results showed that MS medium, 7 g.L-1 agar, 30 g.L-1 sucrose supplemented with 5% coconut water (v/v) were suitable for the regeneration of Breezer, Cerise rosy barbara, Plantom and Red barbara. The shoot number per explants was 5.75
6.00
4.25 and 5.00, respectively. The shoot height was 6.25
6.38
8.00
8.50 (cm), respectively. The leaf number per shoot was 11.75
12.50
12.00
11.75, respectively. The same components of the medium by adding 10% (v/v) coconut water was suitable for Regatta varieties. The shoot number per explants, the shoot height, and the leaf number per shoot were 5.75
8.50
11.25, respectively. MS medium, 7 g.L-1 agar supplemented with 35g sucrose or MS medium, 30 g.L-1 sucrose supplemented with 8 g.L-1 agar were suitable to reduce the vitrification rate of five commercial carnation varieties, while agar 7 g.L-1 or sucrose 30 g.L-1 were suitable for in vitro shoot regeneration. By using UBC808 marker for ISSR-PCR, 5'-G-(AG)7-C-3', it demonstrated that the newly regenerated plant was genetically stable as the mother plant.

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