KHẢO SÁT HIỆU QUẢ BẢO VỆ BỨC XẠ CHO TẾ BÀO LYMPHO NGƯỜI VÀ NGUYÊN BÀO SỢI NGƯỜI CỦA LIPOSOME ĐÓNG GÓI RESVERATROL

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Tác giả: Minh Hiệp Nguyễn, Thị Huỳnh Nga Nguyễn, Ngọc Duy Phạm, Thanh Mai Trần, Thị Ngọc Mai Trần, Ngọc Bích Đào Vũ

Ngôn ngữ: vie

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Thông tin xuất bản: Tạp chí Khoa học và Công nghệ - Đại học Thái Nguyên, 2022

Mô tả vật lý: tr.137-145

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ID: 332161

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Nghiên cứu nhằm mục đích đánh giá hiệu quả bảo vệ bức xạ của liposome đóng gói resveratrol (RES-Lip). Đầu tiên, Lip-RES được tổng hợp bằng phương pháp hydrate hóa màng lipid kết hợp với sóng siêu âm để giảm kích thước. Khả năng xâm nhập của Lip-RES vào tế bào lympho người và nguyên bào sợi người (tế bào HF) được quan sát bằng kính hiển vi huỳnh quang. Kết quả nghiên cứu cho thấy, Lip-RES đã được tổng hợp thành công với kích thước hạt trung bình, chỉ số phân tán, thế zeta, hiệu suất đóng gói, sức tải đạt lần lượt là 103,9 nm, 0,211, -31,9 mV, 92,31%, 8,47%, có dạng hình cầu và có độ bền cao. Lip-RES có khả năng xâm nhập tốt vào tế bào lympho và tế bào HF. Ở điều kiện không chiếu xạ, Lip-RES hầu như không gây độc tính di truyền cho tế bào, tuy nhiên lại gây chết đáng kể cho cả 2 loại tế bào này khi ở nồng độ RES lớn hơn 10 µg/mL. Ở điều kiện chiếu xạ với liều chiếu 2 Gy, Lip-RES giúp làm giảm 16-30% tỉ lệ tế bào chết và giúp làm giảm 10-15% số foci/tế bào, từ đó cho thấy khả năng bảo vệ bức xạ của Lip-RES đối với 2 loại tế bào này. Hơn thế nữa, Lip-RES đạt hiệu quả bảo vệ bức xạ tối ưu ở nồng độ RES 2,5 µg/mL.This study aims to evaluate radioprotection effect of resveratrol-encapsulated liposomes (Lip-RES). Firstly, Lip-RES was prepared by a combining method of lipid-film hydration method and sonication. Penetration of Lip-RES into human lymphocytes and human fibroblasts (HF cells) was observed by fluorescene microscopy. The results demonstrated that Lip-RES was successfully prepared with a relatively spherical shape, good characteristics (mean particle size of 103.9 nm, polydispersity index of 0.211, zeta potential of -31.9 mV, encapsulation efficiency of 92.31% and payload of 8.47%), and a relatively high stability. Lip-RES was well penetrated into human lymphocytes and HF cells. Under non-irradiated condition, Lip-RES showed a negligible genotoxicity, but exhibited a significant cytotoxicity for lymphocytes and HF cells at RES concentration of more than 10 µg/mL. Under irradiated condition at a dose of 2 Gy, compared to the control, Lip-RES could decrease the percentage of cell death of 16-30% and decrease the number of foci/cell of 10-15%. This indicates the radioprotection effect of Lip-RES for human lymphocytes and fibroblasts. Moreover, at a RES concentration of 2.5 µg/mL, Lip-RES showed a best radioprotection.

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