PHÂN TÍCH NHÓM GEN CHÍNH ĐÁP ỨNG VỚI STRESS HẠN VÀ MẶN Ở CÂY ĐẬU GÀ (Cicer arietinum) BẰNG PHÂN TÍCH DỮ LIỆU GIẢI MÃ HỆ PHIÊN MÃ

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Tác giả: Đức Hà Chu, Việt Hồng La, Quốc Trung Nguyễn, Thị Thu Hiền Phan, Văn Hải Tống, Văn Tiến Trần, Thị Lam Hồng Trịnh

Ngôn ngữ: vie

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Thông tin xuất bản: Tạp chí Khoa học và Công nghệ - Đại học Thái Nguyên, 2022

Mô tả vật lý: tr.163-170

Bộ sưu tập: Metadata

ID: 332164

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Mục tiêu của nghiên cứu này nhằm xác định các nhóm gen chính có mức độ biểu hiện khác biệt (DEG) tại mẫu rễ trong điều kiện stress hạn và mặn ở cây đậu gà (Cicer arietinum). Bằng cách khai thác các bộ dữ liệu microarray liên quan đến stress hạn và mặn ở đậu gà, tổng cộng 41 DEG (|fold-change| ≥ 15), bao gồm 15 DEG tăng cường biểu hiện mạnh (fold-change ≥ 15) và 26 DEG kìm hãm biểu hiện mạnh (fold-change ≤ -15) đã được sàng lọc ở mẫu rễ trong điều kiện stress hạn và mặn ở cây đậu gà. Dựa trên hệ tham chiếu của cây đậu gà, phần lớn các DEG được chú giải chức năng mã hóa cho các protein điều hòa và chức năng liên quan đến cơ chế đáp ứng và chống chịu stress thẩm thấu. Phân tích đặc tính lý hóa cho thấy các protein đa dạng về kích thước, trọng lượng phân tử, điểm đẳng điện, độ bất ổn định và độ ưa nước trung bình. Bên cạnh đó, 41 protein được dự đoán cư trú tại nhiều bào quan chính trong tế bào, chủ yếu là ở nhân, tế bào chất và màng sinh chất. Tóm lại, kết quả của nghiên cứu đã cung cấp thông tin về nhóm DEG quan trọng liên quan đến stress hạn và mặn ở rễ cây đậu gà định hướng cho các phân tích chức năng gen.The purpose of this research was to define a core set of differentially expressed genes (DEGs) in roots under the drought and salt stress in chickpea (Cicer arietinum). By exploring all potential microarray datasets related to drought and salt stress in chickpea, a total of 41 DEGs (|fold-change| ≥ 15), including 15 highly up-regulated (fold-change ≥ 15) and 26 highly down-regulated (fold-change ≤ -15) genes was screened in drought- and salt- treated roots. By annotating against the assemblies of chickpea, we found that most of 41 DEG-encoded proteins were annotated as functional and regulatory proteins. Next, our results indicated that 41 DEG-encoded proteins were highly variable in physic-chemical features, like sizes, molecular weights, iso-electric points, instability index and grand average of hydropathy. Furthermore, the prediction of subcellular localization suggested that 41 proteins were localized on many major organelles, especially in the nucleus, cytoplasm and plasma membrane. Taken together, our study could provide a significant core set of DEGs related to drought and salt stress in roots of chickpea for further functional characterization.

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