NGHIÊN CỨU THÀNH PHẦN HÓA HỌC VÀ ĐÁNH GIÁ HOẠT TÍNH SINH HỌC CỦA LOÀI WEIGELA FLORIDA “PINK POPPET”

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Tác giả: Đức Hùng Nguyễn, Văn Khang Phạm, Quang Tâ Từ

Ngôn ngữ: vie

Ký hiệu phân loại:

Thông tin xuất bản: Tạp chí Khoa học và Công nghệ - Đại học Thái Nguyên, 2022

Mô tả vật lý: tr.223-231

Bộ sưu tập: Metadata

ID: 332173

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In this study, qualitative analysis of ethanolic crude extract from the roots of W. florida “Pink Poppet” was investigated. The results revealed that the presence of flavonoid, terpenoid, coumarin and saponin triterpene, but not for alkaloid, steroid and cardiac glycoside. The antioxidant activity was carried out due to the DPPH free radical scavenging resulting in EC50 value of 81.13 µg/mL. The biological activity of this crude extract was further evaluated on cytotoxicity against mouse colon cancer cells (CT26), mouse melanoma cells (B16) and human liver cancer cells (HepG2) by MTS assay. The results displayed the good cytotoxicities of ethanolic crude extract with IC50 values of 19.25
9.16
16.18 µg/mL, in comparison with 5-Fluorouracil as a positive control with IC50 values of 30.23, 16.70, 26.19 µg/mL, respectively. These results suggest a further investigation on isolation and evaluation on chemical compounds from the ethanolic crude extract from roots of W. florida “Pink Poppet”.Trong nghiên cứu này, thành phần hóa học của cao chiết ethanol từ phần rễ của loài W. florida “Pink Poppet” đã được đánh giá qua các phản ứng định tính. Kết quả cho thấy, trong cao chiết có các thành phần flavonoid, terpenoid, coumarin và saponin triterpene, nhưng không có các thành phần alkaloid, steroid và glycoside tim. Cao chiết ethanol đã được xác định có hoạt tính kháng oxy hóa thông qua khả năng loại bỏ gốc tự do DPPH với giá trị EC50 = 81,13 µg/mL. Nghiên cứu sau đó đã đánh giá hoạt tính gây độc trên các dòng tế bào ung thư CT26 (ung thư đại trực tràng), HepG2 (ung thư gan), B16 (ung thư hắc tố da) của cao chiết bằng phương pháp MTS. Kết quả cho thấy, cao chiết ethanol từ phần rễ của loài W. florida “Pink Poppet” có hoạt tính gây độc tế bào ung thư CT26, HepG2 và B16 cao với giá trị IC50 lần lượt là 19,25
9,16
16,18 µg/mL, khi so sánh với đối chứng dương là 5-Fluorouracil với giá trị IC50 lần lượt là 30,23
16,70
26,19 µg/mL. Nghiên cứu đã đề xuất cần tiếp tục nghiên cứu tách chiết và đánh giá hoạt tính sinh học của các hợp chất hóa học có trong cao chiết ethanol từ phần rễ của loài W. florida “Pink Poppet”.

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