TẠO CẤU TRÚC BIỂU HIỆN VÀ BIẾN NẠP GENE GmDREB7A THÔNG QUA AGROBACTERIUM Ở THUỐC LÁ

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Tác giả: Thị Minh Thúy. Vanhsy Sysouphanh Bùi, Hoàng Mậu Chu, Thị Hải Yến Nguyễn, Thị Ngọc Lan Nguyễn, Quang Tân Từ

Ngôn ngữ: vie

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Thông tin xuất bản: Tạp chí Khoa học và Công nghệ - Đại học Thái Nguyên, 2022

Mô tả vật lý: tr.17-25

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ID: 332404

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Đậu tương, loại cây trồng thu hạt có giá trị kinh tế và dinh dưỡng cao và là cây cải tạo đất trồng, nhưng có khả năng chịu hạn, mặn kém. Trong hệ gen đậu tương, nhóm gen DREB được xác định có chức năng kích hoạt phiên mã của các gene chống chịu khi có tín hiệu stress, trong đó có một số gene chưa rõ chức năng cụ thể và DREB7 là một gene trong số đó. Trong bài báo này, gene GmDREB7A và cấu trúc biểu hiện mang gen này được tạo ra và biến nạp thành công vào thuốc lá. Gene GmDREB7A gồm đoạn mã hóa amino acid, đoạn mã hóa c-MYC, KDEL và thêm đoạn nucleotide ngắn ở đầu 5’ và 3’ chứa điểm cắt của enzyme giới hạn. Hai cấu trúc chuyển gen pBI121_GmDREB7A và pZY_GmDREB7A đã được thiết kế thành công. Kết quả biến nạp cấu trúc pBI121_GmDREB7A mang gene GmDREB7A vào thuốc lá đã thu được 248 cây biến nạp sau giai đoạn chọn lọc bằng kanamycin và 30 cây được trồng trong điều kiện nhà lưới với 9 cây dương tính với PCR. Cần tiếp tục phân tích các cây thuốc lá chuyển gen T0 dương tính với PCR và đánh giá khả năng chống chịu các stress hạn, mặn của các cây chuyển gen GmDREB7A.Soybean is a seed crop with high economic and nutritional value and a soil improvement crop that poorly tolerates drought and salinity. In the soybean genome, the DREB gene group has been identified as activating transcription of stress-resistance genes, including some genes of unknown function, and DREB7 is one of them. In this study, the GmDREB7A gene and its expression construct were designed and successfully transformed into tobacco. Gene GmDREB7A includes an amino acid coding sequence, a c-myc coding segment, KDEL, and an additional short nucleotide at the 5,' and 3' ends containing the restriction enzyme's cut-off point. Two transgenic constructs pBI121_GmDREB7A and pZY_GmDREB7A have been successfully designed. The results of structural transformation pBI121_GmDREB7A carrying the GmDREB7A gene into tobacco plants produced 248 transgenic plants after selection with kanamycin, and 30 plants under greenhouse conditions with nine plants were positive for PCR. It is necessary to continue to analyze the T0 transgenic tobacco plants that are positive for PCR and evaluate the tolerance to drought and salinity stress of the GmDREB7A transgenic plants.

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