XÁC ĐỊNH, PHÂN TÍCH CẤU TRÚC VÀ PHÂN NHÓM PROTEIN VẬN CHUYỂN SUCROSE TRONG CÂY CERATOPTERIS RICHARDII

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Tác giả: Đức Hà Chu, Thị Quyến Hà, Việt Hồng La, Thị Yến Linh Nguyễn, Châu Thùy Phạm, Đăng Khoa Trần, Văn Tiến Trần, Thị Thu Thủy Trịnh

Ngôn ngữ: vie

Ký hiệu phân loại:

Thông tin xuất bản: Tạp chí Khoa học và Công nghệ - Đại học Thái Nguyên, 2022

Mô tả vật lý: tr.66-74

Bộ sưu tập: Metadata

ID: 332410

Thêm vào giỏ Liên kết toàn văn

Mục tiêu của nghiên cứu nhằm xác định và phân tích các đặc điểm cơ bản của protein vận chuyển đường sucrose (SWEET) ở cây Ceratopteris richardii, một trong số những loài cây dương xỉ cảnh được trồng trong nhà. Bằng việc sàng lọc vùng bảo thủ đặc trưng, tổng số 20 thành viên của họ protein CericSWEET đã được xác định trong hệ tham chiếu của C. richardii. Kết quả so sánh cho thấy số lượng thành viên của họ SWEET đa dạng giữa các loài thực vật. Phân tích vị trí cư trú nội bào cho thấy các protein CericSWEET cư trú tại hầu hết bào quan thiết yếu. Tiếp theo, nhóm CericSWEET có tính chất lý hóa đa dạng, trong khi hầu hết các gen đều có 6 exon tương tự như ở các loài thực vật khác. Dựa vào sơ đồ hình cây Neighbor-Joining, nhóm CericSWEET gồm 3 phân nhóm. Trong đó, hai gen, CericSWEET07 và 12 được dự đoán có thể có chức năng đáp ứng với điều kiện hạn. Bên cạnh đó, nhiều yếu tố điều hòa cis- đáp ứng hạn và abscisic acid đã được phân tích vùng promoter của các gen CericSWEET. Tóm lại, kết quả của nghiên cứu này đã cung cấp những dẫn liệu quan trọng về nhóm CericSWEET ở C. richardii.The purpose of this research was to identify and characterize the sucrose transporters (SWEET) in Ceratopteris richardii, one of the major ornamental fern houseplants. By screening the conserved domain, a total of 20 members of the CericSWEET family has been identified in the assembly of C. richardii. Our comparison revealed that the numbers of members in the SWEET family were variable between plant species. Subcellular localization showed that CericSWEET proteins were localized in most essential organelles. Next, the CericSWEET family exhibited the variable physic-chemical properties, while a large number of genes contained 6 exons like reported in other plant species. Based on the Neighbor-Joining phylogenetic tree, the CericSWEET family could be classified into 3 sub-groups. Two genes, CericSWEET07 and 12 were hypothesized to function in the drought response. Addtionally, numerous drought- and abscisic acid-responsive cis- regulatory elements have been found in the promoter regions of the CericSWEET genes. Taken together, our results could provide an important foundation for the CerisSWEET family in C. richardii.

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