ẢNH HƯỞNG CỦA MÔI TRƯỜNG CƠ BẢN VÀ CHẤT ĐIỀU HÒA SINH TRƯỞNG ĐẾN SỰ NẢY MẦM HẠT, BIỆT HÓA VÀ TÁI SINH CHỒI LAN HÀI ĐỐM (Paphiopedilum concolor Lindl. Pfitz)

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Tác giả: Tiến Phát Đỗ, Quốc Khánh Nguyễn, Thị Lan Vũ

Ngôn ngữ: vie

Ký hiệu phân loại:

Thông tin xuất bản: Tạp chí Khoa học và Công nghệ - Đại học Thái Nguyên, 2022

Mô tả vật lý: tr.82-90

Bộ sưu tập: Metadata

ID: 332412

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Lan hài Đốm (Paphiopedilum concolor Lindl. Pfitz) là loài Lan hài bản địa của miền Bắc Việt Nam. Hiện nay, loài này đã bị khai thác cạn kiệt ngoài tự nhiên nên việc phát triển phương pháp nhân giống in vitro để bảo tồn là rất cần thiết. Bài báo này trình bày kết quả nghiên cứu về một số yếu tố ảnh hưởng đến hiệu quả nhân giống in vitro Lan hài Đốm bao gồm môi trường khoáng phù hợp cho hạt nảy mầm, môi trường cho phát triển cây con từ hạt, môi trường phù hợp cho tái sinh chồi và sinh trưởng cây con in vitro. Kết quả cho thấy hạt Lan hài Đốm ở độ tuổi 190 ngày (sau thụ phấn) nuôi cấy trên môi trường ½MS hoặc ½VW cho tỷ lệ hạt nảy mầm cao là 82,5% và 80%, thời gian nảy mầm là 37,5 đến 40 ngày. Môi trường phù hợp cho biệt hóa và phát triển của hạt nảy mầm là môi trường P5 (VW + BAP 1,0 mg/L, NAA 0,5 mg/L, chuối 30 g/L, khoai tây 40 g/L) có tỷ lệ tạo chồi đạt cao nhất (50,1%). Môi trường phù hợp cho tái sinh chồi và sinh trưởng của cây con là môi trường VW bổ sung BAP 2,0 mg/L, NAA 0,5 mg/L, số chồi/mẫu đạt 2,0, số lá/chồi đạt 3,5, chiều dài lá đạt 1,35 cm, chồi to, lá xanh đậm và dày.Paphiopedilum concolor (Lindl.) Pfitz is a native species of Northern Vietnam. Recently, this species has been extremely exploited in nature due to deforestation and over-collection, so it is essential to develop in vitro propagation methods for conservation. This paper represent that we have successfully studied a number of factors affecting the efficiency of propagation of Paphiopedilum concolor by in virto seed germination, including suitable mineral medium for seed germination, suitable media added plant growth regulators for differentiation and shoot formation, a suitable medium for shoot regeneration and seedding growth. Seeds were cultured on ½ MS or ½ VW medium gave high germination rates (82.5% and 80%, respectively), germination time was 37.5 to 40 days. The suitable medium for the differentiation and development of germinated seeds was P5 medium (VW + BAP 1.0 mg/L, NAA 0.5 mg/L, banana homogenate 30 g/L, potato homogenate 40 g/L) with the highest percentage of shoot formation (50.1%). The suitable medium for shoot regeneration and seedling growth was VW medium supplemented with BAP 2.0 mg/L, NAA 0.5 mg/L, number of shoots/sample reached 2.0, number of leaves/shoot reached 3.5, leaf length reaches 1.35 cm, seeddings with large, dark green and thick leaves. These results are basis for further studies on in vitro propagation of P. concolor in Vietnam.

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