TẠO DÒNG VÀ GIẢI TRÌNH TỰ TOÀN BỘ GENE E2 CỦA VIRUS DỊCH TẢ HEO CỔ ĐIỂN Ở MỘT SỐ TRẠI HEO TẠI MIỀN NAM VIỆT NAM

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Tác giả: Công Danh Lại, Minh Nam Nguyễn, Ngọc Hải Nguyễn, Thị Phương Bình Nguyễn

Ngôn ngữ: vie

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Thông tin xuất bản: Tạp chí Khoa học và Công nghệ - Đại học Thái Nguyên, 2022

Mô tả vật lý: tr.319-326

Bộ sưu tập: Metadata

ID: 332442

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Bệnh dịch tả heo cổ điển (Classical swine fever disease) là một bệnh lâu đời, nguy hiểm và gây thiệt hại kinh tế đáng kể cho ngành chăn nuôi heo trên toàn thế giới. Trình tự gene mã hóa protein E2 là một cơ sở quan trọng để đánh giá, so sánh đa dạng di truyền của CSFV và phát triển vaccine. Nghiên cứu này được tiến hành nhằm tạo ra các dòng E. coli mang plasmid chứa đầy đủ trình tự gene E2. 21 mẫu dương tính với CSFV đã được thu thập được biến nạp vào các dòng E. coli DH5α. Các plasmid sau đó được tinh sạch và giải trình tự nhằm đánh giá hiệu quả của phương pháp tạo dòng. Kết quả cho thấy nghiên cứu đã tạo thành công 21 dòng vi khuẩn E. coli mang toàn bộ gene E2 của CSFV. Các trình tự này tương đồng cao (87,94 – 98,84%) khi so sánh với 100 trình tự có độ tương đồng cao nhất trên NCBI. Ngoài ra, 19 trình tự tương đồng rất cao với các chủng thuộc phân nhóm 2.1c (95,00 – 96,34%) và 2 trình tự với phân nhóm 2.2 (92,05 – 93,03%). Nghiên cứu này là cơ sở cho việc áp dụng kĩ thuật tạo dòng E. coli mang gene E2 trong việc phân tích di truyền của CSFV một cách ổn định, cũng là cơ sở cho sự tạo ra các protein E2 tái tổ hợp phục vụ việc phát triển vaccine tiểu đơn vị chống CSFV sau này.Classical swine fever is an old and dangerous disease that causes significant economic losses to the swine industry worldwide. E2 protein-coding gene sequencing is an essential basis for assessing and comparing the genetic diversity of CSFV and developing a vaccine against CSFV. This study was conducted to create E. coli cells carrying plasmids containing the full-length E2 gene sequences. Twenty-one CSFV-positive samples were collected that were transformed into E. coli DH5α cells. The plasmids were then purified and sequenced to evaluate the effectiveness of the cloning method. The results showed that the research has successfully created 21 strains of E. coli bacteria carrying the complete E2 gene. These sequences were highly similar (87.94 – 98.84%) compared with the 100 sequences having the highest similarity on NCBI. Besides, 19 sequences had very high similarity with strains belonging to subgroup 2.1c (95.00 - 96.34%) and two sequences with subgroup 2.2 (92.05 - 93.03%). This study is the basis for applying the E. coli cloning technique carrying the E2 gene in stable genetic analysis of CSFV, as well as the basis for the subsequent generation of recombinant E2 proteins for developing an E2-subunit vaccine against CSFV.

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