Xác định đồng thời ethylene oxide và 2-chloroethanol trong thực phẩm bằng sắc ký khí ghép khối phổ ba tứ cực kết hợp với chiết mẫu QuEChERS

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Ngôn ngữ: vie

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Thông tin xuất bản: Tạp chí Kiểm nghiệm và An toàn thực phẩm, 2022

Mô tả vật lý: tr.310-322

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ID: 333574

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In this study, ethylene oxide (EO), a fumigant for agricultural products, and its metabolite (2-chloroethanol, 2-CE), were extracted by QuEChERS method, and then quantified by GC-MS/MS system. Samples were extracted with acetonitrile, and cleaned up with a mixture of dispersive solid phase extraction (d-SPE), including MgSO4, PSA, C18 and GCB, within less than 20 min. The method has been validated on spices, oils, noodle, and food packaging. The results show that this method was high accuracy and precision at spike concentrations, with recovery ranged from 80 to 120%, and RSD lower than 20%. The limit of quantification was 0.01 mg/kg for both compounds, meeting the European (EU) maximum residue limit (MRL) at 0.01 mg/kg. This method has been applied to control the production of instant noodles, with 125 samples of ingredients (seasoning, dried vegetables, oil, flour, food packaging), instant noodles and other food. Among them, 13 ingredients detected 2-CE, and 1 spice detected both compounds, accounting for 11.2%. All 14 samples exceeded the EU MRL.Trong nghiên cứu này, ethylene oxide (EO), một chất hun trùng cho nông sản, và dẫn xuất của nó (2-chloroethanol, 2-CE), được chiết và làm sạch bằng phương pháp QuEChERS, sau đó định lượng trên thiết bị sắc ký khí ghép khối phổ ba tứ cực (GC-MS/MS). Mẫu được chiết bằng acetonitrile, làm sạch bằng kỹ thuật chiết pha rắn phân tán (d-SPE) gồm hỗn hợp MgSO4, PSA, C18, và GCB, với thời gian chuẩn bị mẫu ngắn hơn 20 phút. Phương pháp đã được thẩm định trên các thành phần của gói mì như gia vị, dầu, vắt mì, và bao bì. Kết quả thẩm định cho thấy phương pháp có độ chính xác cao ở các nồng độ khảo sát, với hiệu suất thu hồi từ 80 - 120% và độ lệch chuẩn tương đối (RSD) thấp hơn 20%. Giới hạn định lượng của phương pháp là 0,01 mg/kg cho cả hai chất, hoàn toàn đáp ứng yêu cầu với giới hạn dư lượng tối đa cho phép (MRL) của châu Âu (EU) cho các nền mẫu thực phẩm ở ngưỡng 0,01 mg/kg. Phương pháp này đã được áp dụng cho việc kiểm soát qui trình sản xuất mì gói, với 125 mẫu nguyên liệu (gia vị, rau sấy, dầu, bao bì), mì gói và thực phẩm khác. Trong số đó, có 13 mẫu nguyên liệu phát hiện 2-CE, và 1 mẫu gia vị phát hiện đồng thời cả hai chất, chiếm tỷ lệ 11,2%. Tất cả các mẫu phát hiện đều vượt ngưỡng MRL của EU.

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