Khảo sát khả năng ức chế nấm men của acid sorbic và cyclohoheximide ứng dụng vào quy trình định lượng Lactobacillus spp. trong chế phẩm sinh học probiotic

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Tác giả: Tạp chí Kiểm nghiệm và An toàn thực phẩm

Ngôn ngữ: vie

Ký hiệu phân loại:

Thông tin xuất bản: Tạp chí Kiểm nghiệm và An toàn thực phẩm, 2022

Mô tả vật lý: tr.349-360

Bộ sưu tập: Metadata

ID: 333787

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Khả năng ức chế nấm men của acid sorbic và cycloheximide được chúng tôi tiến hành khảo sát trên 09 chủng nấm men “hoang dại” phân lập từ nhiều nguồn khác nhau. Acid sorbic được bổ sung vào môi trường thạch MRS với hai đồng độ 0,01 g/L (TCVN 5522 : 1991) và 1,4 g/L (TCVN 7906 : 2008). Kết quả cho thấy ở cả hai nồng độ khảo sát, hiệu quả ức chế các chủng nấm men của acid sorbic còn rất hạn chế. Ngược lại, kết quả khảo sát của môi trường thạch MRS bổ sung cycloheximide với dãy nồng độ 0,02
0,04
0,06
0,08 và 0,10 g/L có khả năng ức chế hoàn toàn các chủng nấm men. Hiệu năng của môi trường thạch MRS có bổ sung 0,05 g/L cycloheximide được thử nghiệm với hai thông số độ phát triển (0,7 ≤ PR ≤ 1,4) và hệ số chọn lọc (SF ≥ 2) đạt yêu cầu theo ISO 11133. Kết quả cho thấy khả năng ức chế nấm men của cycloheximide cao hơn so với acid sorbic và đề xuất sử dụng môi trường thạch MRS với hàm lượng 0,05 g/L, pH 6,2 (sau khi hấp khử trùng) để thiết lập qui trình phân tích định lượng Lactobacillus spp. bằng kỹ thuật đổ đĩa với nhiệt độ ủ 37oC ± 1oC trong 72 ± 3 giờ. Bên cạnh đó, dữ liệu nghiên cứu còn góp phần cải tiến quy trình định lượng Lactobacillus spp. nhằm đảm bảo chất lượng kết quả kiểm nghiệm của các trung tâm, đơn vị hoạt động trong lĩnh vực thử nghiệm. Đồng thời nâng cao năng lực, hiệu quả quản lý của cơ quan nhà nước về chất lượng sản phẩm hàng hóa ở Việt Nam nói chung và thành phố Hồ Chí Minh nói riêng.The ability of sorbic acid and cycloheximide to inhibit yeast was investigated on nine yeast strains isolated from different sources. Sorbic acid was added to MRS agar at two concentrations 0.01 g/L (TCVN 5522:1991) and 1.4 g/L (TCVN 7906:2008). The results showed, at both concentrations
the inhibitory effect of sorbic acid on yeast strains was weak. In contrast, the survey results of MRS agar supplemented with cycloheximide with concentration ranges of 0.02
0.04
0.06
0.08
and 0.10 g/L were inhibited yeast strains. The performance testing of MRS agar supplemented with 0.05 g/L of cycloheximide tested with productivity ratio (0.7 ≤ PR ≤ 1.4) and selectivity factor (SF ≥ 2) according to ISO 11133. Therefore, we determined ability to inhibit yeast from cycloheximide was higher than sorbic acid and suggested using MRS agar-supplemented cycloheximide with 0.05 g/L, pH 6.2 (after autoclaving) to establish the quantitative analysis of Lactobacillus spp. by the pouring plate technique, incubated at 37oC ± 1oC for 72 ± 3 hours. In addition, the research results contribute to improving the quantitative analysis of Lactobacillus spp. in order to ensuring the validity of results and providing scientific evidence for decisions on management of effective probiotic quality in Ho Chi Minh City, Vietnam.

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