HOẠT TÍNH ỨC CHẾ VI KHUẨN CỦA CAO CHIẾT NƯỚC TỪ RỄ CÂY DỪA (COCOS NUCIFERA L. VAR TYPICA)

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Tác giả: Đoàn Văn Hậu, Mỹ Lý Phương, Nhụy Trịnh Kiến, Tuấn Nguyễn Minh

Ngôn ngữ: vie

Ký hiệu phân loại:

Thông tin xuất bản: Tạp chí Khoa học - Đại học Thủ Dầu Một, 2022

Mô tả vật lý: tr.72-79

Bộ sưu tập: Báo, Tạp chí

ID: 333944

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Cây dừa có nhiều tác dụng với sức khoẻ đã được ghi nhận trong các tài liệu y học cổ truyền. Nghiên cứu này đánh giá khả năng ức chế sự sinh trưởng của một số chủng vi khuẩn từ cao chiết nước rễ cây dừa (Cocos nucifera L. var typica). Cao chiết nước rễ dừa được làm khô, khảo sát thành phần hoá thực vật, đánh giá khả năng ức chế sinh trưởng trên S. aureus, B. cereus, P. aeruginosa và E. coli và tìm giá trị nồng độ ức chế vi khuẩn tối thiểu (MIC). Cao rễ dừa có chứa hợp chất phenol, tannin, flavonoid, steroid, và saponin. Hoạt tính ức chế vi khuẩn của cao chiết rễ dừa thể hiện sự phụ thuộc vào nồng độ với đường kính vòng vô khuẩn lớn nhất được ghi nhận trên chủng S. aureus (11,67 ± 0,58mm) khi ở nồng độ cao ở 1000mg/ml. Đường kính này đo được trên chủng B. cereus là 8.0 ± 1.0mm. Giá trị MIC được xác định lần lượt là 3,9mg/ml và 15,6mg/ml tương ứng với 2 chủng trên. Ngược lại, không có hoạt tính ức chế của cao đối với E. coli và P. aeruginosa được ghi nhận. Từ kết quả nghiên cứu này, có thể kết luận rằng cao chiết nước rễ dừa có hoạt tính ức chế đối với vi khuẩn gram dương nhưng không có tác dụng đối với nhóm gram âm.The health benefits of the coconut tree have been recorded in traditional medicine. The aim of this study was to evaluate the inhibition activity of water extract of Cocus nucifera L. var typica root on several pathogens. The dried extract was screened for phytochemical components, and the inhibition effect on bacteria was accessed by agar diffusion method. The minimal inhibitory concentration (MIC) was demonstrated by the broth dilution assay. The results showed that the extract contained phenols, tannins, flavonoids, steroids, and saponin. The antibacterial property of the extract was dose-dependent with the zone inhibition diameter increasing with extract concentration. The largest zone inhibition diameter was observed at the concentration of 1000mg/ml of the extract which was 11,67 ± 0,58mm for S. aureus and 8.0 ± 1.0mm for B. cereus. MIC values were 3,9mg/ml and 15,6mg/ml for two above species, respectively. No inhibitory effect of the extract on gram-negative bacteria (E. coli and P. aeruginosa) was found. In conclusion, the water extract of coconut tree root has no antibacterial activity on gram-negative but gram-positive species.

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