Liver cancer and breast cancer have high death rate and increased rapidly in recent years in Vietnam and over the world. Currently, the study of bioactive compounds isolated from plants for killing cancer cells is of interest. Herein, the study aims to identify the cytotoxicity using MTT assay of Combretum quadrangulare Kurz fractions against MCF-7 cells. RT-qPCR method was used to determine the survivin mRNA levels in P12 fraction-treated HepG2 and MCF-7 in comparison to DMSO-treated cells. The results showed that P12 fraction with IC50 of 20.9 ± 0.8 µg/mL was the best cytotoxicity on MCF-7 cells amongst P fractions. P12 fraction exhibited the apoptotic effect on HepG2 and MCF-7 cells by down-regulating survivin expression levels. Thus, P12 fraction was promising for the next research for the impact of fractions assessment in vivo or bioactive compounds isolation.