ĐÁNH GIÁ SỰ DUY TRÌ VÀ BIỂU HIỆN GEN ZmLEA14A Ở CÂY NGÔ CHUYỂN GEN

0 Người đánh giá. Xếp hạng trung bình 0

Tác giả: Trường Sơn Đinh, Thị Thu Huệ Huỳnh, Thùy Linh Nguyễn

Ngôn ngữ: vie

Ký hiệu phân loại:

Thông tin xuất bản: Tạp chí Khoa học và Công nghệ - Đại học Thái Nguyên, 2023

Mô tả vật lý: tr.78-84

Bộ sưu tập: Metadata

ID: 334297

Thêm vào giỏ Liên kết toàn văn

Maize is one of the important crops which is widely used in biology and genetic research. The LEA gene family was predicted to have 32 members and was classified into 9 groups including: LEA1, LEA2, LEA3, LEA4, LEA5, LEA6, SMP, Dehydrin, and AtM. The Lea14A gene is among the group 5C, the protein Lea14A could help cell resistance for plants. Therefore, the enhancement of Lea14A gene expression may increase drought tolerance in plants that has potential for agricultural applications.This study was conducted to evaluate the presence of transgene and its expression at RNA and protein levels in ZmLea14A transgenic maize from T0 to T2 generation. K7 variety maize was used for transferring ZmLEA14A gene via A. tumefaciens. The results showed that the ZmLEA14A gene was stably transmitted to the T2 generations. Gene expression and protein level of ZmLEA14A were demonstrated by RT-PCR and Western hybridization. The transgenic lines would be a useful materials for further studies to create drought tolerant maize.Ngô là cây lương thực chính trong nông nghiệp nước ta và được nghiên cứu ứng dụng cũng như nghiên cứu cơ bản khá nhiều. Họ LEA được dự đoán gồm 32 gen trên toàn hệ gen ngô và bao gồm 9 nhóm: LEA1, LEA2, LEA3, LEA4, LEA5, LEA6, SMP, Dehydrin và AtM. Protein do gen Lea14A mã hóa nằm trong số các protein LEA nhóm 5C và là protein có chức năng giúp tăng khả năng chống chịu của tế bào. Vì vậy, việc tăng cường biểu hiện gen ZmLea14A nhằm mục đích tăng chống chịu cho cây, hứa hẹn tạo tính trạng tốt cho cây trồng nói chung. Nghiên cứu này được thực nhiện nhằm đánh giá sự có mặt của gen ZmLea14A, sự phiên mã và dịch mã của gen ZmLea14A ở cây ngô chuyển gen. Sử dụng giống ngô K7 làm vật liệu để chuyển gen ZmLEA14A nhờ vi khuẩn A. tumefaciens mang cấu trúc 35S-ZmLEA14A. Kết quả cho thấy gen ZmLEA14A duy trì ổn định qua các thế hệ T0 đến T2. Sự phiên mã và dịch mã của gen ZmLEA14A được chứng minh bằng các phương pháp RT-PCR và lai Western. Các cây ngô chuyển gen ZmLEA14A sẽ được sử dụng cho các nghiên cứu tiếp theo nhằm tạo dòng ngô chịu hạn.

1. Đọc trực tuyến

1. Đọc trực tuyến

Tạo bộ sưu tập với mã QR