BIẾN NẠP VÀ ĐÁNH GIÁ CÁC DÒNG XOAN TA (Melia azedarach L.) MANG GEN GA20-OXIDASE ĐƯỢC ĐIỀU KHIỂN BỞI PROMOTER GmPrP2

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Tác giả: Tiến Phát Đỗ, Việt Hồng La, Khánh Linh Lý, Xuân Phong Ong, Bích Ngọc Phạm

Ngôn ngữ: vie

Ký hiệu phân loại:

Thông tin xuất bản: Tạp chí Khoa học và Công nghệ - Đại học Thái Nguyên, 2023

Mô tả vật lý: tr.113-120

Bộ sưu tập: Metadata

ID: 334302

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Cây xoan ta là một cây lâm nghiệp có nhiều công dụng, ở Việt Nam cây xoan được trồng khá phổ biến. Trong nghiên cứu này cấu trúc mang gen GA20ox mã hoá cho enzyme GA20-oxidase đóng vai trò quan trọng trong quá trình sinh tổng hợp GA ở tế bào thực vật đã được chuyển vào cây xoan ta bằng vector GmPrP2:GA20ox. Kết quả kiểm tra sự có mặt của gen GA20ox bằng kỹ thuật PCR có 15 dòng mang gen. Các dòng xoan mang gen chuyển được đánh giá mức độ biểu hiện ở cấp độ phân tử bằng kỹ thuật RT-PCR cho kết quả gen GA20ox đã hoạt động. Đánh giá sinh trưởng các dòng mang gen chuyển sau 8 tuần tuổi thì dòng T16, T20 và T22 có chiều cao và số đốt tăng 150 - 200% so với đối chứng. Giải phẫu đốt thân thứ 8 cho thấy mật độ sợi xylem và số tế bào trên sợi xylem của các dòng mang gen chuyển đều cao hơn dòng đối chứng, còn độ dày thành tế bào các dòng tương tự nhau. Những kết quả này bước đầu chứng minh vai trò của promoter GmPrP2 với gen GA20ox đối với quá trình tăng trưởng và cải thiện sinh khối ở cây thân gỗ.Melia azedarach L. is a forest tree with many uses, in Vietnam it is grown quite popularly. In this study, GA20 encoding for the enzyme GA20-oxidase which plays an important role in GA biosynthesis in plant cells was transferred to the plant by vector GmPrP2:GA20ox.. The results of checking the presence of the GA20ox gene by PCR showed that there were 15 lines carrying the gene. Transgene-carrying sinus lines were evaluated for expression at the molecular level by RT-PCR and also showed that the GA20ox gene was active. Assessing the growth of transgenic lines after 8 weeks of age, lines T16, T20 and T22 had an increase in height and number of nodes by 150 - 200% compared to the control. Anatomy of the 8th stem showed that the density of xylem fibers and the number of cells on the xylem fibers of the transgenic lines were higher than that of the control lines, and the cell wall thickness of the lines were similar. These results demonstrate the role of the GmPrP2 promoter with the GA20ox gene on growth and biomass improvement in woody plants.

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