Định lượng đồng thời 1-[(2-chlorophenyl) methyllimino methyl] cyclopentanol hydroclorid và 2-Hydroxy-2- (o-chloro phenyl) cyclohexanon, hai tiền chất của ketamin, trong nước thải bằng LC-MS/MS

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Thông tin xuất bản: Tạp chí Kiểm nghiệm và An toàn thực phẩm, 2022

Mô tả vật lý: tr.451-461

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Ketamin is a pharmaceutical compound that is abused as a narcotic drug, causing health problems for users. In controlling ketamine abuse, the detection of ketamine precursors can be a useful tool for tracing illegal ketamine production sites. In this study, an analytical method for determination of two ketamine precursors, 1-[(2-chlorophenyl) methyllimino methyl] cyclopentanol hydroclorid (CCM) and 2-Hydroxy-2- (o-chloro phenyl) cyclohexanon (HCH), in wastewater using solid phase extraction (SPE) and liquid chromatogrphy tandem mass spectrometry (LC-MS/MS) was developed and validated. An LC-MS/MS system employing a Zorbax C18 column (2.1 x 100 mm, 1.8 µm) and a mobile phase composing acetonitrile and water containing 0.1% (m/v) of formic acid in a gradient program at the flow rate of 0.3 mL/min and injection volume of 5 µL. The detection of analytes was done with electrospray ionization at positive mode (ESI+) employing multi reaction monitoring (MRM) at m/z of 238.5 and 225.5 for for CCM and HCH, respectively. The method was validated according to requirements of AOAC International and EC and was proved as reliable for intended use.Ketamin là một dược chất đã được lạm dụng như một ma tuý, có thể gây nhiều tác hại cho sức khoẻ người sử dụng. Trong kiểm soát ketamin, việc phát hiện các tiền chất trong quy trình sản xuất ketamin là một công cụ hữu hiệu trong việc khoanh vùng và triệt phá các cơ sở sản xuất ma tuy trái phép. Trong nghiên cứu này, phương pháp định lượng hai tiền chất của ketamin là 1-[(2-chlorophenyl) methyllimino methyl] cyclopentanol hydroclorid (CCM) và 2-hydroxy-2- (o-chloro phenyl) cyclohexanon (HCH) trong nước thải sử dụng kỹ thuật chiết pha rắn (SPE) kết hợp với sắc ký lỏng khối phổ hai lần (LC-MS/MS) đã được xây dựng và thẩm định. Phương pháp phân tích sử dụng cột Zorbax C18 (2,1 × 100 mm, 1,8 µm) và pha động gồm hỗn hợp acetonitril- nước chứa acid formic 0,1% (kl/tt) theo chương trình gradient với lưu lượng 0,3 mL/phút và thể tích tiêm mẫu 5 µL. Chất phân tích được phát hiện bằng ion hoá phun điện tử ion dương (ESI+) và chế độ theo dõi đa phản ứng (MRM) ở m/z 238,5 và 225,5 cho các ion mẹ CCM và HCH, và m/z 220,1 và 125,2 cho các ion con định lượng của CCM và HCH. Phương pháp được thẩm định theo hướng dẫn của AOAC đạt yêu cầu cho việc ứng dụng trong định lượng hai chất này trong mẫu nước thải.

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