ẢNH HƯỞNG CỦA CHẤT ĐIỀU HOÀ SINH TRƯỞNG THỰC VẬT ĐẾN QUÁ TRÌNH NHÂN GIỐNG IN VITRO CÂY BA KÍCH (Morinda officinalis How.)

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Tác giả: Ngọc Hoàng Vân Nguyễn, Trọng Tuấn Trần, Thị Hương Trịnh

Ngôn ngữ: vie

Ký hiệu phân loại:

Thông tin xuất bản: Tạp chí Khoa học Công nghệ và Thực phẩm (Tên mới: Tạp chí Khoa học Đại học Công Thương), 2022

Mô tả vật lý: tr.37

Bộ sưu tập: Metadata

ID: 335804

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In this study, the effect of plant growth regulators on micropropagation of Morinda officinalis through nodal explants was investigated. The results showed that Murashige and Skoog medium supplemented with 1.5 mg/L benzyladenine (BA) combined with 0.25 mg/L kinetin (KIN) was a suitable medium for the rapid multiplication of shoots. On this medium, the number of shoots was 4.1 shoots per nodal explant after 4 weeks of culture. For rooting, indole-3-butyric acid (IBA) was a more suitable auxin than naphthaleneacetic acid (NAA) because of the formation of callus at the base of the cultured shoots on media using NAA. At the concentration of 0.5 mg/L IBA, the maximum rate of rooting explants, number of roots per explant, and average height were 100%, 6.2 roots/explant, and 5.8 cm after 6 weeks of culture, respectively. The plantlets were acclimatized in the greenhouse with a 93.3% survival rate after 4 weeks. These results contribute to the development of a stable micropropagation protocol of M. officinalis, which can be successfully used for the production of plantlets for the medicinal plant market in Vietnam as well as for the conservation of this important medicinal plant.Trong nghiên cứu này, ảnh hưởng của một số chất điều hoà sinh trưởng thực vật đến quá trình vi nhân giống cây ba kích (Morinda officinalis How.) từ đoạn đốt thân của cây in vitro được thực hiện. Kết quả thu được cho thấy, môi trường Murashige và Skoog (MS) bổ sung 1,5 mg/L benzyladenine (BA) kết hợp với 0,25 mg/L kinetin (KIN) là môi trường thích hợp cho quá trình nhân nhanh chồi, với số lượng chồi mới tạo thành 4,1 chồi/đốt thân sau 4 tuần nuôi cấy. Đối với giai đoạn ra rễ, việc bổ sung indole-3-butyric acid (IBA) vào môi trường nuôi cấy thích hợp hơn so với naphthaleneacetic acid (NAA). Các chồi nuôi cấy trên môi trường bổ sung NAA có sự hình thành mô sẹo ở phần gốc. Ở nồng độ IBA 0,5 mg/L, tỷ lệ mẫu ra rễ, số rễ/mẫu và chiều cao cây trung bình đạt được cao nhất, tương ứng là 100%, 6,2 rễ/mẫu và 5,8 cm sau 6 tuần nuôi cấy. Các cây con sinh trưởng tốt ở điều kiện vườn ươm, với tỷ lệ sống sót là 93,3% sau 4 tuần trồng. Các kết quả đạt được của nghiên cứu này góp phần xây dựng một quy trình vi nhân giống cây ba kích ổn định, có thể cung cấp nguồn cây giống cho thị trường cây dược liệu ở Việt Nam cũng như ứng dụng vào bảo tồn nguồn gen của loài cây dược liệu quý này.

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