TỐI ƯU HÓA ĐIỀU KIỆN TÁCH CHIẾT SAPONIN TRITERPENOID TỪ BÃ HẠT CÂY SỞ (Camellia oleifera) BẰNG PHƯƠNG PHÁP ĐÁP ỨNG BỀ MẶT (RSM)

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Tác giả: Thị Mai Trinh Đỗ, Thị Hạnh Nguyễn, Thị Liên Nguyễn, Minh Ngọc Trương

Ngôn ngữ: vie

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Thông tin xuất bản: Tạp chí Khoa học Công nghệ và Thực phẩm (Tên mới: Tạp chí Khoa học Đại học Công Thương), 2022

Mô tả vật lý: tr.76

Bộ sưu tập: Metadata

ID: 335811

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Mục tiêu của nghiên cứu là khảo sát một số yếu tố ảnh hưởng đến quá trình chiết xuất saponin triterpenoid từ bã hạt cây Sở (Camellia oleifera) bằng dung môi ethanol. Sự ảnh hưởng của các yếu tố bao gồm: nồng độ dung môi (ethanol), tỷ lệ dung môi/ nguyên liệu (v/w) và thời gian tách chiết lần lượt được khảo sát. Hàm lượng saponin triterpenoid được xác định bằng phương pháp đo quang phổ UV-Vis sau khi chiết mẫu và làm phản ứng tạo màu Rosenthaler của saponin với thuốc thử acid perchloric và vanillin. Thực hiện tối ưu hóa các điều kiện chiết xuất theo phương pháp đáp ứng bề mặt Response Surface Method (RSM) kết hợp với mô hình tâm phức hợp (CCD). Bã hạt cây Sở được thu mua từ cơ sở ép dầu Quang Thanh, tỉnh Nghệ An, sau đó được sấy khô ở nhiệt độ 105 ºC trong 5 giờ được dùng cho các thí nghiệm. Kết quả tối ưu theo mô hình CCD cho thấy hàm lượng saponin triterpenoid đạt cực đại 5,451% tại tỷ lệ dung môi: nguyên liệu là 19:1 (v/w), nồng độ EtOH 80% và thời gian 77 giờ. Kết quả thực nghiệm cho kết quả có độ tương thích cao với mô hình.The study aimed to investigate some of factors affecting the extraction of triterpenoid saponins from the residue of seeds of Camellia oleifera by ethanol solvent. The influence of factors including solvent concentration (ethanol), material/solvent ratio, and extraction time were investigated in turn. The content of triterpenoid saponins was determined by UV-Vis spectrophotometric method after sample extraction and rosenthaler coloration reaction of saponins with reagents of perchloric acid and vanillin in acetic acid gave the product a lilac purple color. Optimization of extraction conditions was performed by Response Surface Method (RSM) combined with the complex center model (CCD). The residue of seeds of Camellia oleifera was purchased from the local oil press at Quang Thanh facility, Nghe An province, then dried at 105 ºC for 5 hours, was used for the experiments. According to the CCD model, the optimal results showed that the triterpenoid saponin content 5.451% at the liquid-solid ratio 19: 1 (v/w), the EtOH concentration 80%, and the time 77 hours. The experimental results show high compatibility with the model

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