Khảo sát hoạt tính sinh học của các cao chiết từ cây Bạch hoa xà thiệt thảo (Hedyotis diffusa Willd., Rubiaceae)

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Tác giả: Thị Thu Hiền Nguyễn

Ngôn ngữ: vie

Ký hiệu phân loại:

Thông tin xuất bản: Tạp chí Khoa học và Công nghệ - Đại học Nguyễn Tất Thành, 2022

Mô tả vật lý: tr.17

Bộ sưu tập: Báo, Tạp chí

ID: 335842

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Nghiên cứu tiến hành nhằm sơ bộ thành phần hóa học thực vật, chiết xuất, phân tách các cao phân đoạn từ cây Bạch hoa xà thiệt thảo, khảo sát hoạt tính sinh học của các cao chiết bao gồm chống oxi hóa bằng phương pháp DPPH, kháng viêm in vitro, gây độc tế bào trên dòng tế bào ung thư người. Từ 3 kg Bạch hoa xà thiệt thảo khô được ngấm kiệt với cồn 70 %, cô giảm áp thu được 60 g cao toàn phần. Lắc phân bố cao lỏng thu được 30,1 g cao petroleum ether, 38,6 g cao chloroform, 20,4 g cao ethyl acetat. Các mẫu cao đều có khả năng chống oxi hóa và yếu hơn so với mẫu đối chứng dương acid ascorbic, trong đó cao E có hoạt tính mạnh nhất với giá trị IC50 là 30,52 µg/mL. Cao toàn phần có khả năng ức chế NO trên tế bào RAW264.7 tốt nhất (IC50 <
30 µg/mL) . Tuy nhiên các mẫu cao đều gây độc tế bào RAW264.7 mạnh. Thử nghiệm trên tế bào ung thư gan – HepG2 và ung thư phổi – A549 cho thấy cao chloroform có hoạt tính gây độc tế bào tốt nhất với giá trị IC50 lần lượt là (81,16 và 92,72) µg/mL. Kết quả chỉ ra các hoạt chất trong cây Bạch hoa xà thiệt thảo có tác dụng chống oxi hóa, kháng viêm, gây độc tế bào và là loại dược liệu có tiềm năng.This research aims at understanding the chemical composition, extracting, and isolating fragmented gels from Hedyotis diffusa, then surveying on the gels’ antioxidizing effects using DPPH analysis, on their anti-inflammatory effectts in vitro, and on their cytotoxic effects on human cancer cells. From 3kg of dried Hedyotis diffusa, after soaking with 700 alcohol and then concentrating a total of 60 g gross extracts (cao T) was collected. After distribution shaking process of the liquid, 30.1 g petroleum ether extract (cao P), 38.6 g chloroform extract (cao C), and 20.4 g ethyl acetate extract (cao E) were collected. All had antioxidizing effects, though weaker than the compared ascorbic acid. Of the three, cao E had the highest effects with IC50 value at 30.52 µg/mL. Cao T has the best NO inhibition on RA264.7 cell (IC50 <
30 µg/mL). However, all test gels had strong cytotoxic effects on RAW 264.7. The results on liver cancer cells – HepG2 – and lung cancer cells – A549 – showed that cao C had the best cytotoxic effects with IC50 values being 81.16 and 92.72 µg/mL perspectively. The results proved the potentials of the antioxidizing, anti-inflammatory and cytotoxic effects of the extracts, prompting the applications of Hedyotis diffusa in Medicine.

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