Khảo sát thành phần hóa học cao ethyl acetate của cây Quyển bá trường sinh (Selaginella tamariscina)

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Tác giả: Thị Châu Ngô

Ngôn ngữ: vie

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Thông tin xuất bản: Tạp chí Khoa học và Công nghệ - Đại học Nguyễn Tất Thành, 2022

Mô tả vật lý: tr.22

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ID: 335844

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Cây Quyển bá trường sinh thuộc chi Selaginella thu hái ở tỉnh Quảng Ngãi được nghiên cứu với mục đích tìm ra thành phần hóa học trong cao ethyl acetate bằng cách phân lập và xác định cấu trúc các thành phần hóa học. Từ 7,3 kg mẫu cây Quyển bá trường sinh (Selaginella tamariscina), bằng kĩ thuật trích Soxlet lần lượt với các dung môi n-hexane, ethyl acetate và methanol, thu hồi dung môi cho ra các cao chiết tương ứng. Thực hiện sắc kí cột kết hợp với sắc kí bản mỏng cao chiết ethyl acetate (50,2 g) trên silica gel pha thường, pha đảo RP-18 và Sephadex LH-20 với những hệ dung môi khác nhau, hai hợp chất tinh khiết đã được phân lập. Dựa trên phổ 1H và 13C NMR kết hợp so sánh với tài liệu tham khảo, cấu trúc của các hợp chất này đã được xác định, bao gồm: một dẫn xuất chromone, 5,7-dihydroxy-2,6,8-trimethylchromone (1) và một dẫn xuất glycoside steroid, stigmast-5,22-dien-3-O-β-D-glucopyranoside (2). Đây là lần đầu hai hợp chất này được tìm thấy trong cây Quyển bá trường sinh ở Việt Nam. Những phân đoạn còn lại cũng đang được tiếp tục nghiên cứu thành phần hóa học để phân lập thêm những hợp chất khác.Selaginella tamariscina was picked in Quang Ngai province and studied with the aim of finding out the chemical compositions in ethyl acetate solvent by isolating and determining the structure of its chemical component. Extraction of Selaginella tamariscina (7.3 kg) using Soxlet method with the solvents of n-hexane, ethyl acetate and methanol in turn gave corresponding extracts after vaporization of the solvents. Column chromatography and thin layer chromatography of the ethyl acetate extract (50.2 g) on silica gel, RP-18 and Sephadex LH-20 with different solvent systems led to isolation of two compounds. Based on data of 1H and 13C NMR, as well as comparison of literature, the structure of these compounds were identified as a chromone, 5,7-dihydroxy-2,6,8-trimethylchromone (1) and a steroidal glycoside, stigmast-5,22-dien-3-O-β-D-glucopyranoside (2). This is the first time these compounds have been found in Selaginella tamariscina in Vietnam and the world. Furthermore, chemical study of remaining fractions are being carried out to collect more compounds in order to test for biological activity such as anticarcinogenic, anti-oxidant or anti-inflammatory.

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