Seven compounds including abietatriene (1), oleanolic acid (2), quercetagetin 3,7-dimethyl ether (3), luteolin (4), p-hydroxybenzoic acid (5), p-methylbenzoic acid (6), and protocatechuic acid (7) were isolated from the n-hexane: ethyl acetate (1:1) extract of Vitex negundo leaves collected in Binh Thuan Province. Their chemical structures were elucidated by comparing their spectroscopic data with reported data in the literature. Six compounds (exception of 4) were known to be present in Vitex negundo.