ĐÁNH GIÁ KHẢ NĂNG PHÂN HỦY DIOXIN CỦA MỘT SỐ CHỦNG VI KHUẨN PHÂN LẬP TỪ ĐẤT Ô NHIỄM TẠI SÂN BAY A SO (HUẾ)

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Tác giả: Minh Trí Lê, Thị Tâm Thư Nguyễn, Văn Hoàng Nguyễn, Kiên Cường Phạm

Ngôn ngữ: vie

Ký hiệu phân loại:

Thông tin xuất bản: Tạp chí Khoa học và Công nghệ - Đại học Thái Nguyên, 2023

Mô tả vật lý: tr.335 - 342

Bộ sưu tập: Báo, Tạp chí

ID: 337087

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A So Airport is one of the hot spots for dioxin contamination because of the war. Biodegradation of dioxin isomers by microorganisms is an environmentally friendly method, capable of restoring contaminated land. In this study, from dioxin-contaminated soil samples at A So Airport (Thua Thien Hue), 9 strains of microorganisms capable of degrading persistent organic compounds were isolated. In which, 2 strains capable of degrading all 17 dioxin isomers at high concentrations were identified. The AS2 strain belongs to the genus Klebsiella and is named Klebsiella sp. AS2, whose registration number on GenBank is ON890383. The AS3 strain belongs to the genus Bacillus and is named Bacillus sp. AS3, whose registration number on GenBank is ON890397. The two strains were able to degrade 17 dioxin isomers with the efficiency from 1.47% to 100% after 30 days depending on the isomer type. Total toxicity in culture samples of 2 strains AS2 and AS3 decreased by 71.83% and 41.73%, respectively, with total initial toxicity of 3000 pg/ml. The AS2 and AS3 strains degraded 2,4-D with an efficiency of 68.61% and 52.32%, respectively, with an initial concentration of 200 ppm. Also with this concentration, the two strains degraded 2,4,5-T with the rates of 72.14% and 59.73%.Sân bay A So là một trong các điểm nóng về ô nhiễm dioxin do hậu quả của chiến tranh. Phân hủy sinh học các đồng phân dioxin bởi vi sinh vật là phương pháp thân thiện môi trường, có khả năng phục hồi vùng đất ô nhiễm. Trong nghiên cứu này, từ các mẫu đất ô nhiễm dioxin tại sân bay A So (Thừa Thiên Huế), 9 chủng vi sinh vật có khả năng phân hủy các hợp chất hữu cơ khó phân hủy được phân lập. Trong đó có 2 chủng có khả năng phân hủy cả 17 đồng phân dioxin ở nồng độ cao được định danh. Chủng AS2 thuộc chi Klebsiella và được đặt tên là Klebsiella sp. AS2, có mã số đăng ký trên GenBank là ON890383. Chủng AS3 thuộc chi Bacillus và được đặt tên là Bacillus sp. AS3, có mã số đăng ký trên GenBank là ON890397. Hai chủng có khả năng phân hủy 17 đồng phân dioxin với hiệu suất từ 1,47 % đến 100% sau 30 ngày tùy từng loại đồng phân. Tổng độ độc trong mẫu nuôi cấy 2 chủng AS2 và AS3 giảm tương ứng là 71,83% và 41,73% với tổng độ độc ban đầu là 3000 pg/ml. Chủng AS2 và AS3 phân hủy 2,4-D với hiệu suất 68,61% và 52,32% tương ứng với nồng độ 2,4-D ban đầu là 200 ppm. Cũng với nồng độ này, hai chủng phân hủy 2,4,5-T với tỷ lệ 72,14% và 59,73%.

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