ẢNH HƯỞNG CỦA VIỆC BỔ SUNG HUYẾT THANH THAI BÒ TRONG MÔI TRƯỜNG NUÔI CẤY ĐẾN SỰ PHÂN LẬP VÀ TĂNG SINH TẾ BÀO NGUYÊN SỢI TỪ MẪU MÔ BUỒNG TRỨNG HEO Lưu Khải Nhiên1 và Nguyễn Ngọc Tấn *

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Tác giả: Khoa học Kỹ thuật Chăn nuôi

Ngôn ngữ: vie

Ký hiệu phân loại:

Thông tin xuất bản: Tạp chí Khoa học Kỹ thuật Chăn nuôi, 2023

Mô tả vật lý: tr.45662

Bộ sưu tập: Metadata

ID: 337114

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Effect of Fetal bovine serum supplementation in culture media on isolation and proliferation of fibroblast cells from porcine ovarian tissue The aim of this study was to investigate to effect of fetal bovine serum (FBS) supplemented in culture medium for fibroblast cells from porcine ovarian tissue. Tissue explants were submitted to primary culture in medium with different concentration of FBS for 8 days with a medium change at day 4. Results shown that between the two treatments of supplementing 10 and 20% FBS throughout 8 days, a significant difference (5.01 and 5.57
P<
0.05) of cells harvested was found. No differences of harvested cell numbers were observed between supplementing 10-20 and 20-10% at first 4 days - later 4 days (5.46 and 5.40, respectively). Three levels of FBS concentration (10, 15 and 20%) were evaluated for subculture medium, proliferative activity of cells were recorded to build a growth curve and determine population doubling time (PDT). There were significant differences (P<
0.05) between 10 and 20% FBS supplementation in culture medium on day 3 (4.04 and 4.24, respectively), day 5 (4.24 and 4.45, respectively) and day 7 (4.19 and 4.41, respectively). No differences were recorded between the 15 and 20% treatments. The PDT of 10, 15 and 20% treatments was 68.89, 66.65 and 62.04 hours, respectively. The growth curve with the exponential phase spanning from day 1 to day 5 was determined. In conclusion, a supplementation of 10% FBS in the first 4 days and 20% FBS in the later 4 days of primary culture medium and subculture in medium with 15% FBS enhanced the total number of fibroblast cells isolated and proliferated from porcine ovarian tissue.Nghiên cứu nhằm khảo sát sự ảnh hưởng của việc bổ sung huyết thanh thai bò (FBS-fetal bovine serum) ở các nồng độ khác nhau trong môi trường nuôi cấy tế bào đến sự hình thành và tăng sinh các tế bào nguyên sợi từ mô buồng trứng heo. Các mảnh mô buồng trứng được nuôi cấy sơ cấp trong môi trường DMEM có bổ sung FBS ở các nồng độ khác nhau, được nuôi trong 8 ngày và thay môi trường vào ngày thứ 4. Kết quả cho thấy số lượng tế bào thu được sau 8 ngày nuôi cấy ở môi trường có nồng độ 10% FBS thấp hơn so với 20% FBS (5,01 so với 5,57
P<
0,05). Ở 2 nghiệm thức bổ sung 10 và 20% FBS lần lượt cho 4 ngày đầu và 4 ngày sau và ngược lại, không có sự khác biệt ý nghĩa (5,46 và 5,40
P>
0,05) và không khác biệt so với nghiệm thức bổ sung 20% FBS trong suốt 8 ngày nuôi cấy. Nuôi tăng sinh của tế bào trong môi trường có nồng độ FBS là 10, 15 và 20%, kết quả cho thấy có sự khác biệt ý nghĩa (P<
0,05) về lượng tế bào thu được giữa 2 mức nồng độ 10 và 20% FBS ở ngày 3 (lần lượt là 4,04 và 4,24), ngày 5 (lần lượt là 4,24 và 4,45) và ngày 7 (lần lượt là 4,19 và 4,41). Tuy nhiên, không có sự khác biệt giữa việc bổ sung 15 và 20% vào môi trường nuôi thứ cấp. Thời gian phân chia của 3 mức bổ sung 10, 15 và 20% lần lượt là 68,89
66,65 và 62,04 giờ. Đường cong tăng trưởng của tế bào nuôi cấy trong môi trường chứa nồng độ FBS khác nhau cho thấy lượng tế bào đạt cực đại vào ngày 5. Vì vậy, bổ sung FBS ở mức 10% trong 4 ngày đầu và 20% trong 4 ngày sau vào môi trường nuôi cấy sơ cấp và bổ sung 15% FBS vào môi trường nuôi cấy thứ cấp giúp cải thiện được lượng tế bào nguyên sợi phân lập và tăng sinh được từ mô buồng trứng heo.

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