Ảnh hưởng giai đoạn bổ sung yếu tố tăng trưởng nội mao mạch trong môi trường nuôi thành thục tế bào trứng đến sự thành thục nhân và phát triển của phôi đơn tính ở heo thu từ nang noãn nhỏ

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Tác giả: Khoa học Kỹ thuật Chăn nuôi

Ngôn ngữ: vie

Ký hiệu phân loại:

Thông tin xuất bản: Tạp chí Khoa học Kỹ thuật Chăn nuôi, 2023

Mô tả vật lý: tr.45883

Bộ sưu tập: Báo, Tạp chí

ID: 337117

Thêm vào giỏ Liên kết toàn văn

Mục tiêu của nghiên cứu này là đánh giá ảnh hưởng của thời điểm bổ sung yếu tố tăng trưởngnội mao mạch (VEGF) trong môi trường nuôi cấy thành thục (IVM) đến tỷ lệ thành thục nhân vàphát triển phôi đơn tính từ nguồn tế bào trứng heo thu nhận từ nang noãn nhỏ. Phức hợp tế bàotrứng-cumulus (COC) có từ 2 lớp cumulus trở lên sau khi được thu nhận từ nang noãn nhỏ (SF)được lựa chọn đưa vào nuôi cấy với các thời điểm bổ sung VEGF khác nhau trong IVM. Kết quảcho thấy tỷ lệ thành thục về nhân của tế bào trứng thu từ MF cao hơn so SF khi không bổ sungVEGF (74,6 và 45,4%
P<
0,05). Ở các thời điểm bổ sung VEGF khác nhau, tỷ lệ thành thục về nhâncao nhất khi bổ sung VEGF ở nhóm tế bào trứng thu từ SF trong 22 giờ đầu IVM (73,9%), tiếp đếnlà 44 giờ (71,2%), sau đó là 22 giờ sau (58,5%) và được ghi nhận là thấp nhất khi không bổ sungVEGF (45,4%). Đặc biệt, khi bổ sung VEGF ở nhóm tế bào trứng SF trong suốt 44 giờ và 22 giờ đầucủa IVM cho kết quả về tỷ lệ thành thục nhân không khác biệt có ý nghĩa so với nhóm tế bào trứngthu từ MF khi không bổ sung VEGF (74,6%
P>
0,05). Sau quá trình IVM, các tế bào trứng có xuấthiện thể cực thứ nhất được kích hoạt phôi đơn tính bằng ethanol 7% kết hợp với 2,5µM 6-DMAP.Tỷ lệ phân chia tế bào và hình thành phôi nang khi bổ sung VEGF trong giai đoạn suốt 44 giờ (lầnlượt là 70,7 và 24,4%) và 22 giờ đầu (lần lượt là 74,3 và 28,3%) của IVM ở nhóm tế bào trứng SF khácbiệt không có ý nghĩa so với nhóm tế bào trứng thu từ MF khi không bổ sung VEGF (79,9 và 31,7%
P>
0,05). Vì vậy, việc bổ sung VEGF ở nồng độ 250 ng/ml vào môi trường ở 22 giờ đầu hoặc suốt 44giờ của IVM không những giúp cải thiện tỷ lệ thành thục nhân mà còn giúp nâng cao hiệu quả sảnxuất phôi đơn tính của tế bào trứng heo thu từ nang noãn nhỏ trong điều kiện in vitro.Stage-dependent effect of Vascular Endothelial Growth Factor protein in the matuarationmedium on nuclear maturation and developmental competence of parthenogenetic embryos ofporcine oocytes derived from small folliclesThis study aimed to evaluate the stage-dependent effect of VEGF protein supplementationin maturation medium on nuclear maturation and further parthenogenetic embryos developmentof porcine oocytes derived from small follicles. Cumulus-Oocyte Complexes (COCs) with morethan 2 layers were collected from medium follicles (MF) and small follicles (SF) for in vitromaturation (IVM) in 44hrs, the oocyte with the first polar body was subjected to parthenogeneticactivation by ethanol. Result showed that the nuclear maturation rate of MF-derived oocytes wassignificantly higher than SF-derived oocytes in condition of without VEGF supplementation (74.6and 45.4%, P<
0.05). Furthermore, the result also indicated that the stage-dependent effect of VEGFsupplementation in maturation medium on nuclear maturation, cleavage rate and blastocystformation of SF-derived oocytes was found. The percentage of nuclear maturation was highest inSF-derived oocytes supplemented with VEGF during the first 22hrs of IVM (73.9%) as comparedto the 44hr-treated group (71.2%), last-22hr-treated group (58.5%) and non-treated group (45.4%).Meanwhile, no significant differences between non-treated MF-derived (74.6%, P>
0.05), first-22hrand 44hrs group after IVM were observed. After parthenogenetically activation was applied, thecleavage and blastocysts rate in SF-derived oocytes of the 44hr-treated group (70.7 and 24.4%,respectively) and first-22hr-treated group (74.3 and 28.3%, respectively) also showed no significantdifferences to MF group (79.9 and 31.7%, respectively, P>
0.05). In conclusion, supplementing 250ng/ml of VEGF during the first 22hrs or entire 44hrs of IVM not only enhance the nuclear maturationbut also increase the cleavage rate and blastocyst formation of oocytes derived from small follicles.

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