Quy trình nuôi cấy dòng tế bào thường trực PIPEC để gây nhiễm virus vacxin nhược độc dịch tả lợn châu Phi chủng SFV-G-DELTA-I177L/DELTA LVR

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Tác giả: Duy Kháng Đinh, Bá Hiên Nguyễn, Như So Nguyễn, Thế Tường Nguyễn, Thị Thanh Nga Nguyễn, Thị Thu Hường Nguyễn, Thị Hằng Phạm, Thị Hòa Phạm, Đăng Đồng Vũ

Ngôn ngữ: vie

Ký hiệu phân loại:

Thông tin xuất bản: Tạp chí Khoa học kỹ thuật Thú y, 2023

Mô tả vật lý: tr.15

Bộ sưu tập: Báo, Tạp chí

ID: 337486

Thêm vào giỏ Liên kết toàn văn

African swine fever is a highly dangerous and contagious disease, which has appeared in Viet Nam since 2019 and caused heavy losses for the pig industry. A major challenge in the production of African swine fever vaccines is that there is no a stable, suitable cell line for a vaccine candidate virus to replicate on an industrial scale. ASFV-G-Delta I177L/Delta LVR attenuated candidate vaccine virus strain generated by deleting the I177L and LVR gene has been investigated, this virus strain has the ability to adapt on a very stable porcine-derived PIPEC cell line (Plum Island Porcine Epithelial Cells). This study was conducted with the aim of standardizing the process of PIPEC cell culture (PIPIC – trade name for export), experimental infection with ASFV-G-Delta I177L/Delta LVR attenuated vaccine strain leading to research on the production process of attenuated vaccines against African swine fever for the pig herd in Viet NamBệnh dịch tả lợn châu Phi là bệnh truyền nhiễm nguy hiểm, đã xuất hiện ở Việt Nam từ năm2019 và gây thiệt hại nặng nề cho ngành chăn nuôi lợn. Thách thức lớn trong sản xuất vacxin dịchtả lợn châu Phi là chưa tìm được dòng tế bào thích ứng cho virus - ứng cử viên vacxin - nhân lên ởquy mô công nghiệp. ASFV-G-ΔI177L/ΔLVR, một chủng virus ứng viên nhược độc được tạo ra từviệc xoá gen I177L và vùng LVR đã được nghiên cứu và có khả năng thích ứng trên một dòng tế bàothường trực có nguồn gốc từ lợn rất ổn định là dòng tế bào thường trực PIPEC (Plum Island Porcine Epithelial Cells). Nghiên cứu này được thực hiện nhằm chuẩn hoá quy trình nuôi cấy tế bào PIPEC (PIPIC – tên thương mại xuất khẩu), gây nhiễm thử nghiệm chủng virus vacxin nhược độc ASFV-GΔI177L/ΔLVR tiến tới nghiên cứu quy trình sản xuất vacxin nhược độc phòng bệnh dịch tả lợn châu Phi cho đàn lợn ở Việt Nam

1. Đọc trực tuyến

1. Đọc trực tuyến

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