Một số đặc tính sinh học và tính sinh miễn dịch của chủng virus porcine epidemic diarhea phân lập tại miền Bắc Việt nam

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Tác giả: Bùi Tiến Hoàng, Đức Lưu Nguyễn, Thanh Ba Nguyễn, Thị Bích Nguyễn, Văn Khánh Trần

Ngôn ngữ: vie

Ký hiệu phân loại:

Thông tin xuất bản: Tạp chí Khoa học kỹ thuật Thú y, 2023

Mô tả vật lý: tr.22

Bộ sưu tập: Metadata

ID: 337488

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Bài báo này được trình bày dựa trên số liệu nghiên cứu bốn chủng virus porcine epidemic diarrhea(PEDV) đã được phân lập tại miền Bắc Việt Nam, nhằm tuyển chọn chủng virus dùng trong nghiên cứu,sảnxuất vacxin phòng bệnh tiêu chảy cấp (PED).Các chủng PEDVthực địa đã được giám định genogroup bằngkỹ thuậtmultiplexRT-PCR. Kết quả nghiên cứu cho thấy các chủng virusthực địa thuộc genogroups 2, trongkhi virus vacxin nhược độc (chủng SM98) từ vacxin nhập khẩu hiện đang lưu hành ở Việt Nam lại thuộcgenogroup 1. Khi nhuộm miễn dịch tế bào vero nhiễm PEDV thực địa đã quan sát thấy, các tế bào vero đơn lẻ nhiễm virus chiếm đa số, thể hợp bào hình thành nhỏ, hiệu giá virus cao nhất đạt 106,1TCID50. Nghiên cứu tính sinh miễn dịch giữa các chủng virus đã được thực hiện bằng phản ứng trung hòa virus, kết quả cho thấy chủng PEDV 0118 thực địa có khả năng kháng chéo 100% với 3 chủng thực địa còn lại trong nghiên cứuThis article is presented basing on the database of four porcine epidemic diarrhea virus (PEDV) strains which were studied, isolated in the Northern Viet Nam with the aim of selecting a PEDV strain for studying and producing vaccines against PEDV. Four field PEDV strains were identified for their genogroup by multiplex RT-PCR technique. The studied results showed that these field strains belonged to genogroups 2, while the attenuated vaccine strain from the imported vaccine that currently using in Viet Nam belonged to genogroup 1. The replication of these field PEDV strains in the vero cells were observed by immunostaining, the virus strains were infected into single cells to be majority and syncytial was small, virus titre reaching 106.1TCID50 was the highest. Immunogenicity studies between virus strains were performed by viral neutralization. As a result, PEDV 0118 field strain was 100% cross-resistant with three field strains left.

1. Đọc trực tuyến

1. Đọc trực tuyến

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