In this study, we introduce a thin film composite (TFC) nanofiltration (NF) membrane prepared by the interfacial polymerization (IP) using dopamine-biomonomer with trimesoyl chloride (TMC). The cellulose acetate (CA) ultrafiltration membrane was used as a support layer. Dopamine can not only firmly adhere to the support layer but also can form a uniform polyamide layer to improve water transport and separation. The fabricated TFC NF properties were characterized using modern techniques, including attenuated total reflectance-Fourier transform infrared spectroscopy (ATRFTIR), scanning electron microscopy (SEM), and contact angles. Dopamine-based TFC NF membrane exhibited a high permeability of 48.2 L m-2 h-1 bar-1 and a congo red rejection of 99.3%.