In this study, the silver nanoparticles (AgNPs) were successfully synthesized via a simple, nontoxic and ecofriendly method by using tea leaf extract. The formation of AgNPs was identified by changing the color of the reaction medium from light yellow to dark brown and the surface plasma resonance band. The optimal conditions for the synthesis of silver nanoparticles were determined by changing various parameters including pH, amount of extract, concentration of AgNO3 solution and time. Furthermore, the biosynthesized AgNPs were characterized by Fourier transform infrared spectroscopy (FT-IR), Xray diffraction (XRD) and scanning electron microscopy (SEM). The AgNPs were nearly spherical in shape with the crystalline size of about 10 nm. The active compounds in the extract containing functional groups such as hydroxyl, amine, ether acted as a reducing agent to convert silver ions into AgNPs and capping agent on the surface of AgNPs. Silver nanoparticles exhibited significant antibacterial activity against P. aeruginosa and S. aureus with the inhibition zones ranging between 19-26 mm and 22-29 mm, respectively.