ẢNH HƯỞNG CỦA ĐIỀU KIỆN THỦY PHÂN ĐẾN KHẢ NĂNG TRÍCH LY CATECHIN TRONG VỎ LỤA HẠT ĐIỀU VỚI SỰ HỖ TRỢ CỦA CÁC CHẾ PHẨM ENZYME VISCOZYME L VÀ PECTINEX ULTRA-L

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Tác giả: Nguyễn Huỳnh Đạt Duy, Văn Thảnh Hoàng, Thanh Tùng La, Xuân Hoàn Nguyễn, Văn Hùng Phạm

Ngôn ngữ: vie

Ký hiệu phân loại:

Thông tin xuất bản: Tạp chí Khoa học Công nghệ và Thực phẩm (Tên mới: Tạp chí Khoa học Đại học Công Thương), 2023

Mô tả vật lý: tr.40

Bộ sưu tập: Metadata

ID: 338312

Thêm vào giỏ Liên kết toàn văn

Nghiên cứu này với mục tiêu tìm ra điều kiện thủy phân thích hợp để thu được hàm lượng catechin tổng có trong dịch chiết từ vỏ lụa hạt điều cao nhất bằng phương pháp trích ly có hỗ trợ enzyme (EAE: Enzyme-assisted extraction). Hai chế phẩm enzyme khác nhau là Viscozyme L và Pectinex ultra-L được sử dụng với các yếu tố khảo sát gồm: nồng độ enzyme, tỷ lệ nguyên liệu/dung môi, nhiệt độ và thời gian thủy phân. Kết quả nghiên cứu cho thấy khi thủy phân bằng Viscozyme L với các điều kiện nồng độ enzyme 2%
tỷ lệ nguyên liệu/dung môi 1:30 (w/v)
nhiệt độ 50oC
thời gian 60 phút, hàm lượng catechin tổng tương ứng là 16,09 ± 0,73 (g CE/100g chất khô mẫu). Đối với Pectinex ultra-L ở các điều kiện nồng độ enzyme 2%
tỷ lệ nguyên liệu/dung môi 1:20 (w/v)
nhiệt độ 50oC
thời gian 60 phút hàm lượng catechin tổng đạt được là 19,98 ± 0,50 (g CE/100g chất khô mẫu). Ngoài ra, kết quả cũng chỉ ra rằng các hoạt chất sinh học như flavonoid, khả năng bắt gốc tự do 1,1-diphenyl-2- picrylhydrazyl (DPPH) của mẫu dịch chiết sử dụng Pectinex ultra-L cao hơn so với khi sử dụng Viscozyme L hay mẫu đối chứng không sử dụng enzyme. Như vậy, kết quả của nghiên cứu này cho thấy phương pháp trích ly có hỗ trợ enzyme Pectinex ultra-L cho hiệu quả trích ly catechin từ vỏ lụa hạt điều cao hơn so với phương pháp có hỗ trợ Viscozyme L hoặc không có enzymeThe objectives of this study are to investigate appropriate hydrolyzation conditions to obtain the highest amount of catechin of the cashew nut testa using the enzyme-assisted extraction method. Two types of enzymes, Viscozyme L and Pectinex ultra-L, were used to study with different conditions including enzyme concentration, sample/buffer ratios, temperature, and incubation time. The results indicated that the highest catechin concentration (16.09 ± 0.73 g CE/100g dried sample) was obtained when Viscozyme L was used to assist the extraction with the concentration of 2%, sample/buffer ratio of 1/30, the temperature of 50oC, and incubation time of 60 min. Regarding the Pectinex ultra-L, the appropriate conditions of enzyme concentration of 2%, sample/buffer ratio of 1/20 (w/v), the temperature of 50oC, and incubation time of 60 min were determined to obtain the highest catechin content of 19.98 ± 0.50 (g CE/100 g dried sample). Moreover, the results also indicated that flavonoid content and DPPH radical scavenging capacity of the extracts from cashew nut testa using Pectinex ultra-L were higher than those of the extracts using Viscozyme L or the control. Thus, the extraction using the Pectinex ultra-L was more effective than that using the Viscozyme L or without enzyme.

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