PHÂN LẬP VÀ XÁC ĐỊNH MỘT SỐ NẤM MỐC GÂY HẠI TRÊN VỎ TRÁI THANH LONG Hylocereus costaricensis SAU THU HOẠCH

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Tác giả: Phan Thị Kim Uyên Đặng, Thị Thu Huyền Nguyễn, Bùi Minh Tâm Phạm, Thị Mai Trinh Trần

Ngôn ngữ: vie

Ký hiệu phân loại:

Thông tin xuất bản: Tạp chí Khoa học Công nghệ và Thực phẩm (Tên mới: Tạp chí Khoa học Đại học Công Thương), 2023

Mô tả vật lý: tr.49

Bộ sưu tập: Metadata

ID: 338329

The dragon fruit is a fruit with high nutritional, economic value for Vietnam but it has a short shelf life. Harmful mold is one of the causes of post-harvest quality loss of dragon fruit. This study was conducted to find out harmful mold strains on the dragon fruit peel Hylocereus costaricensis during post-harvested storage. By inoculating nine damaged peel samples of the dragon fruit H. costaricensis from a dragon fruit garden in Long An province on the selective agar media Czapeck-Dox, five mold strains were isolated and purified. Basing on the macroscopic (colony morphology) and microscopic (cell morphology observed by light microscope) characteristics, strains N1 and N2 could belong to genus Fusarium, strain N3 could belong to genus Neoscytalidium, strain N4 could belong to the genus Rhizopus and strain N5 could belong to the genus Aspergillus. Two mold strains N1 and N3 were further identified as Fusarium fujikuroi (100% similarity) and Neoscytalidium sp. (100% similarity) by sequence analyses of ITS and LSU regions of rRNA gene in combination with morphological characteristics, respectively. The artificial infection examination showed that both F. fujikuroi N1 and Neoscytalidium dimidiatum N3 strains caused the damages on fruits after harvest. The obtained results of this study provided more data of harmful molds, thereby helping to guide the prevention and preservation of post-harvested dragon fruit Hylocereus costaricensis in Viet Nam.The objectives of this study are to investigate appropriate hydrolyzation conditions to obtain the highest amount of catechin of the cashew nut testa using the enzyme-assisted extraction method. Two types of enzymes, Viscozyme L and Pectinex ultra-L, were used to study with different conditions including enzyme concentration, sample/buffer ratios, temperature, and incubation time. The results indicated that the highest catechin concentration (16.09 ± 0.73 g CE/100g dried sample) was obtained when Viscozyme L was used to assist the extraction with the concentration of 2%, sample/buffer ratio of 1/30, the temperature of 50oC, and incubation time of 60 min. Regarding the Pectinex ultra-L, the appropriate conditions of enzyme concentration of 2%, sample/buffer ratio of 1/20 (w/v), the temperature of 50oC, and incubation time of 60 min were determined to obtain the highest catechin content of 19.98 ± 0.50 (g CE/100 g dried sample). Moreover, the results also indicated that flavonoid content and DPPH radical scavenging capacity of the extracts from cashew nut testa using Pectinex ultra-L were higher than those of the extracts using Viscozyme L or the control. Thus, the extraction using the Pectinex ultra-L was more effective than that using the Viscozyme L or without enzyme.
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