In aviation, safety is the highest priority. In order to yield socio-economic benefits, there is a need to carry out aviation activities safely. To ensure the aviation safety, the system and technical aspects are not enough, human factors and organizational culture have been drawn more and more attention. Thus, there is increasing interest in applying the concept of safety culture in aviation industry. Using the measurement of safety culture proposed by Association of Asia Pacific Airlines, Arab Air Carriers Organization and Airport Council International (ICAO, 2015), this study sets out to investigate the safety culture in a wide range of aviation sectors in the context of Vietnam. From March 1st to April 15th, 2019, 350 random employees in various aviation functional areas are involved in the survey measuring aviation safety culture. A number of quantitative techniques are utilized for testing the measurement and processing the collected data. The findings of the assessment reveal a generally good existing safety culture in aviation sector. However, some aspects of safety culture (e.g. communication, reporting, and commitment) are somewhat poorer than that estimated at some functional areas. In addition, safety risk management & reporting has positive relationship with overall assessment of safety culture amongst employee with 95% confidence. Although the safety culture measurement proposed by ICAO (2015) is perceived as useful for exploring and highlighting safety assessment in Vietnam, further work is required. This will involve the integration with different measurements developed and verified by other researches, and considering the national culture to ensure that it operates consistently in local settin