Marchantia polymorpha has been associated with many pharmaceutical applications in Asian countries, such as treating inflammation, liver disease, open wounds, boils, fractures, insect and snake bites, etc. This study investigated the phytochemical of M. polymorpha grown in Lam Dong Province. The acetone extract of M. polymorpha was applied to silica gel column chromatography to obtain five compounds. Their chemical structures were elucidated by using Nuclear Magnetic Resonance spectroscopy, as well as by the comparison of their NMR data with reported ones. Five compounds were elucidated as (Z)-dimethomorph (1), (E)-dimethomorph (2), methyl 3,4-dihydroxybenzoic acid (3), marchantin A (4), and quercetin (5).