Khảo sát khả năng của phycocyanin trong bảo vệ da in vitro và in vivo

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Tác giả: Lại Đình Biên, Loan Đặng Hồng, Lê Thành Long, Trang Hoàng Ngọc Ngân, Tô Minh Quân, Uyên Lê Thị Thu

Ngôn ngữ: vie

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Thông tin xuất bản: Tạp chí khoa học Đại học Mở Thành phố Hồ Chí Minh - Kỹ thuật và Công nghệ, 2023

Mô tả vật lý: tr.57-66

Bộ sưu tập: Báo, Tạp chí

ID: 339075

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Phycocyanin (PC) is an antioxidative protein with blue color. PC occupies 10% of the biomass of Arthrospira platensis. In this study, we aimed to investigate the ability of PC to protect skin from harmful agents. Human fibroblasts (hF) were incubated in the culture medium containing PC (0.1 - 10 µg/ml) one day before treating with 150µM H2O2 for 90 minutes. Four days after treatment, cell shape and expression of a biomarker of senescence, senescence-associated β-galactosidase (SA-gal), were determined. Mus musculus var. albino was used in in vivo experiment. Shaved dorsal skin was topically applied with PC 50 µg/ml 03 hours prior to UV irradiation. The treatment was performed 06 times per week for 08 weeks. After that, skin texture and histology were determined. The results showed that 10 µg/ml PC relieved the harmful effect of H2O2 on hF. In this group, the hF remained elongated shape similar to in the control group and the expression of SA-gal in cells was lower than in the H2O2 group (27.1 ± 6.1%). After 08 weeks, dorsal skin treated with PC prior to UV treatment was still smooth with fewer wrinkles, and the skin’s structure was similar to the control. Conclusion: phycocyanin can protect skin from environmental factors in vitro and in vivo.Phycocyanin (PC) là một protein màu xanh lam có hoạt tính chống oxi hóa. PC chiếm khoảng 10% khối lượng khô tảo Arthrospira platensis. Hiện nay, chưa có nhiều nghiên cứu đánh giá hiệu quả sử dụng PC trong lĩnh vực mỹ phẩm. Trong nghiên cứu này, PC được khảo sát khả năng bảo vệ tế bào da trong điều kiện in vitro và in vivo. Đối với thử nghiệm in vitro, tế bào nguyên bào sợi người (hF) được nuôi trong môi trường DMEM/F12 chứa PC (0.1 -10 µg/ml) 24 giờ trước khi xử lý với 150µM H2O2 trong 90 phút. Sau 04 ngày, tiến hành khảo sát hình dạng tế bào và xác định marker đặc trưng cho quá trình lão hóa senescence-associated β-galactosidase (SA-gal). Đối với thử nghiệm trên chuột, chuột Mus musculus var. albino được cạo sạch da lưng, bôi PC 50 µg/ml 03 giờ trước mỗi lần chiếu UV. Thí nghiệm được tiến hành trong 08 tuần, mỗi tuần xử lý PC và chiếu UV 06 ngày. Sau 08 tuần, đánh giá tính chất bề mặt da và nhuộm mô học vùng da xử lý. Kết quả cho thấy PC nồng độ 10 µg/ml đã làm giảm tác hại của H2O2 đối với tế bào: tế bào vẫn duy trì dạng thon dài, tỉ lệ diện tích nhân/tế bào tương tự như tế bào ban đầu (0.16 ± 0.06), tỉ lệ tế bào biểu hiện SA-gal thấp (27.1 ± 6.1%). Sau 08 tuần xử lý, da chuột được bôi với PC 50 µg/ml trước khi chiếu UV có tính chất: mịn, ít nếp nhăn, cấu trúc mô học da tương tự như da tự nhiên. Kết luận: phycocyanin các tác dụng bảo vệ nguyên bào sợi da in vitro khỏi tác nhân hydrogen peroxide ở nồng độ 10 µg/ml và in vivo khỏi tác hại của tia UV ở nồng độ 50 µg/ml.

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