Thiết kế mồi gene cho phản ứng Nested-MSP khuếch đại gene RASSF1A (Ras association domain family member 1)

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Tác giả: Đạt Nguyễn Thành, Huệ Thiều Hồng, Ngô Đông Kha, Nguyễn Thị Thu Thảo, Thuận Lao Đức, Lê Huyền Ái Thúy, Nguyễn Ngọc Toàn, Trần Thị Quế Trân

Ngôn ngữ: vie

Ký hiệu phân loại:

Thông tin xuất bản: Tạp chí khoa học Đại học Mở Thành phố Hồ Chí Minh - Kỹ thuật và Công nghệ, 2023

Mô tả vật lý: tr.75-83

Bộ sưu tập: Metadata

ID: 339078

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Methylation of Tumor Suppressor Genes (TSGs) has been identified as the cause of nasopharyngeal tumorigenesis. Among TSGs, RASSF1A plays an important role in cell cycle regulation, microtubule stablilization, and control ostabilization invasion and metastasis. The methylation occurs on the gene of RASSF1A, which has been reported as the early event of nasopharyngeal carcinoma. Therefore, in this study, the primers for Nested-MSP method will be designed based on bioinformatics tools: Methprimer, IDT, and Annbyb. As the result, primer pairs, including sequence primers, methylation primers, and unmethylation primers, were successfully designed. The length of the PCR assay with these primers were 392bp, 200bp và 173bp, respectively. Thus, this study provides the primer pairs for the Nested-MSP method to further investigate the methylation of RASSF1A gene in nasopharyngeal carcinoma.Tính chất methyl hóa các gene ức chế khối u là một trong những nguyên nhân gây nên ung thư vòm họng. RASSF1A là một gene ức chế khối u đóng vai trò quan trọng trong điều hòa chu kì tế bào, điều chỉnh sự ổn định của các vi ống và có khả năng kiểm soát sự xâm lấn và di căn. Trong nghiên cứu, các cặp mồi sử dụng cho phương pháp Nested-MSP sẽ được thiết kế dựa trên các công cụ tin sinh học: Methprimer, IDT và Annhyb. Kết quả chúng tôi đã thiết kế thành công các cặp mồi, bao gồm: mồi ngoài, mồi methyl và mồi unmethyl sử dụng cho phản ứng Nested-MSP khuếch đại sản phẩm PCR với kích thước lần lượt là 392bp, 200bp và 173bp. Như vậy, từ nghiên cứu này cung cấp cặp mồi cho phản ứng Nested-MSP hướng tới khảo sát tính chất methyl hóa của gene RASSF1A trên Ung Thư Vòm Họng (UTVH).

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