Khảo sát độc tính cấp, khả năng kháng oxi hóa và kháng viêm của cao Trầu không (Piper betle l. Piperaceae)

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Tác giả: Thái Quỳnh Thi Bùi, Thị Phương Liên Hoàng, Thị Quỳnh Nhi Lê, Nhựt Trường Nguyễn, Thị Bạch Tuyết Nguyễn

Ngôn ngữ: vie

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Thông tin xuất bản: Tạp chí Khoa học và Công nghệ - Đại học Nguyễn Tất Thành, 2023

Mô tả vật lý: tr.19

Bộ sưu tập: Báo, Tạp chí

ID: 339082

Thêm vào giỏ Liên kết toàn văn

Cao Trầu không đã được tiêu chuẩn hóa, được chiết xuất với dung môi cồn 96 % và nước. Hoạt tính kháng oxi hóa của cao Trầu không được xác định theo phương pháp khử gốc tự do DPPH. Thử nghiệm in vivo được thực hiện trên chuột nhắt trắng Swiss albino, (6-8) tuần tuổi, trọng lượng trung bình khoảng 22 g. Khảo sát độc tính cấp đường uống của cao. Chuột được uống cao Trầu không với liều duy nhất 5.000 mg/kg trọng lượng chuột với thể tích 50 mL/kg trọng lượng chuột, theo dõi tỉ lệ chết và biểu hiện độc tính cấp trong vòng 14 ngày. Hiệu quả kháng viêm của cao Trầu không với liều (200, 400 và 800) mg/kg được đánh giá gây viêm bàn chân chuột nhắt bằng carrageenan 1 %. Diclofenac liều 5 mg/kg được sử dụng làm chất đối chứng. Kết quả cho thấy cao Trầu không thể hiện hoạt tính kháng oxi hóa với IC50 là 8,25 µg/mL, kém 3 lần so với quercetin. Cao Trầu không gây ra độc tính cấp đường uống ở nồng độ 5.000 mg/kg, được xếp vào phân loại 6 – chất gần như không có độc tính theo GSH. Ở mô hình gây viêm bằng carragenan, cao Trầu không thể hiện tác động làm giảm độ phù chân chuột đáng kể ở liều 200 mg/kg (p <
0,05) và tương đương với diclofenac liều 5 mg/kg (p >
0,05)The study used standardized betel leaf extract with 96 % alcohol and water. For in vitro study, antioxidant capacity of betel’s extract was performed by DPPH method - free radical scavenging. In vivo testing was performed on Swiss albino mice, (6-8) weeks aged, with an average weight of about 22 g. The investigation of acute oral toxicity was performed at 5,000 mg/kg dosage with a volume of 50 mL/kg of rat, monitoring mortality and toxicity within 14 days. The anti-inflammatory effect of extract at doses of (200, 400 and 800) mg/kg was evaluated in mice induced with paw inflammation with 1 % carrageenan. Diclofenac 5 mg/kg was used as a drug reference. The results showed that betel’s extract showed an antioxidant activity with IC50 of 8.25 µg/mL, about 3 times worse than quercetin. This extract does not cause acute oral toxicity at a concentration of 5,000 mg/kg, and is classified in category 6 – a substance that is almost non-toxic according to GSH (Globally Harmonised System for Classification of Chemicals). In the carragenan-induced inflammation model, this extract showed a significant reduction in paw edema in mice at (200, 400 and 800) mg/kg doses (p <
0.05).

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