Khảo Sát Hoạt Tính Kháng Oxy Hóa, Kháng Khuẩn Và Kháng Nấm Từ Dịch Chiết Cây Rau Quế Vị (Limnophila Rugosa (Roth) Merr.)

0 Người đánh giá. Xếp hạng trung bình 0

Tác giả: Ngọc Khôi Phạm

Ngôn ngữ: vie

Ký hiệu phân loại:

Thông tin xuất bản: Tạp chí Y Dược học Phạm Ngọc Thạch, 2023

Mô tả vật lý: tr.109

Bộ sưu tập: Metadata

ID: 339149

Thêm vào giỏ Liên kết toàn văn

- Đặt vấn đề: Cây rau quế vị hay còn gọi là cây hồi nước, quế đất có tên khoa học là Limnophila rugosa (Roth) Merr... Cây thuộc họ Hoa mõm sói (Scrophulariaceae) phân bố chủ yếu ở Ấn Độ, Nepal, Myanmar, Trung Quốc, Thái Lan, Lào, Việt Nam. Cây được trồng làm rau gia vị và là một loại thảo dược phòng được nhiều bệnh như suy nhược cơ thể, trị cảm, viêm họng, trị ho, viêm phế quản, đau dạ dày hay trị mụn.- Mục tiêu nghiên cứu: Xác định một số hoạt tính sinh học như kháng oxy hóa, kháng khuẩn, kháng nấm từ dịch chiết toàn phần từ cây rau quế vị.- Đối tượng và phương pháp nghiên cứu: Toàn thân cây rau quế vị được tách chiết bằng phương pháp ngấm kiệt (dung môi ethanol 70%, tỷ lệ nguyên liệu - dung môi là 1:10 (g/mL), 60 phút, 50ºC) để thu được cao đặc toàn phần. Cao đặc sau đó được pha loãng thành dịch chiết toàn phần theo tỷ lệ 1:1 với dung môi ethanol 70%. Sử dụng dịch chiết toàn phần này để khảo sát tiếp tác dụng kháng oxy hóa (dựa vào tác dụng khử gốc tự do của 2,2-diphenyl-1-picrylhydrazyl) và tác dụng kháng khuẩn, kháng nấm (bằng phương pháp đặt đĩa kháng sinh).- Kết quả: Dịch chiết toàn phần từ cây rau quế vị có tác dụng kháng oxy hóa cao (IC50 = 25,53 µg/ml), nhưng vẫn thấp hơn vitamin C (IC50 = 10,24 µg/ml) là mẫu đối chứng. Dịch chiết toàn phần có tác dụng kháng lại một số chủng vi khuẩn (Escherichia coli, Pseudomonas aeruginosa, Staphylococcus epidermidis, Streptococcus pneumoniae) và vi nấm (Candida albicans) ở nhiều nồng độ khảo sát (40 - 80 mg/ml). Ở nồng độ dịch chiết toàn phần càng cao thì hoạt tính kháng khuẩn, kháng nấm càng tăng dần. Tuy nhiên, dịch chiết toàn phần này không có tác dụng kháng được vi khuẩn Acinetobacter baumannii ở tất cả các nồng độ khảo sát.- Kết luận: Lần đầu tiên tác dụng kháng oxy hóa, kháng khuẩn, kháng nấm của dịch chiết toàn phần từ cây rau quế vị (Limnophila rugosa (Roth) Merr.) đã được chứng minh. Kết quả của nghiên cứu này mở ra hướng nghiên cứu liên quan đến các tác dụng trên của cây rau quế vị, từ đó làm cơ sở cho các nghiên cứu sâu hơn về hoạt tính sinh học khác của cây rau quế vị.Abstract- Background: Limnophila rugosa (Roth) Merr.belongs to the family Scrophulariaceae, distributed mainly in India, Nepal, Myanmar, China, Thailand, Laos and Vietnam. This plant is vegetable andis a herb that prevents many diseases such as weakness, cold treatment, sore throat, cough, bronchitis, stomachache or acne.- Aim: The aim of this study is to demonstratesome biological activities such as antioxidant, antibacterial, and antifungal activities of whole extracts from Limnophila rugosa (Roth) Merr..- Material and method: Limnophila rugosa (Roth) Merr.were totally extracted by extraction conditions (ethanol 70%, raw materials: ethanol (1:10 g/mL), 60 minutes, 50ºC). The extract was then diluted to solution. Use this solution to study the antioxidant activities (based on the ability to eliminate free radicals of 2,2-diphenyl-1-picrylhydrazyl)
antibacterial andantifungal activities (by antibiotic disc set).- Result: This study evaluated the ability to capture free radicals DPPH of Limnophila rugosa (Roth) Merr.with IC50 value (25.53 μg/ml), whereas vitamin C (10.24 μg/ml). In this study, whole extracts capable of inhibiting the expression of Escherichia coli, Pseudomonas aeruginosa, Staphylococcus epidermidis, Streptococcus pneumoniae, Candida albicans (40 - 80 mg/ml). At the higher concentration of the extract, the antibacterial and antifungal activities increase gradually. However, this extract does not have the ability to resist bacteria such as Acinetobacter baumanniiat all investigated concentrations.- Conclusion: For the first time, some biological activitiesas antioxidant, antibacterial, antifungal of Limnophila rugosa (Roth) Merr.extracts were investigated. The results of this study help supplement therapeutic potentials for further studies on other biological activities. DOI: 10.59715/pntjmp.1.1.14

1. Đọc trực tuyến

1. Đọc trực tuyến

Tạo bộ sưu tập với mã QR