PHÂN LẬP VI KHUẨN LACTIC CÓ HOẠT TÍNH ĐỐI KHÁNG NẤM Colletotrichum sp. GÂY BỆNH THÁN THƯ TRÊN TRÁI ỚT SỪNG SAU THU HOẠCH

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Tác giả: Thị Hồng Gấm Nguyễn, Trí Yến Chi Nguyễn, Văn Cao Thi Quách, Quốc Dũng Trần

Ngôn ngữ: vie

Ký hiệu phân loại:

Thông tin xuất bản: Tạp chí Khoa học và Công nghệ - Đại học Thái Nguyên, 2023

Mô tả vật lý: tr.349-356

Bộ sưu tập: Metadata

ID: 339692

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Anthracnose caused by Colletotrichum sp. appear more popular, causing much damage to the yield and quality of many crops. Therefore, this study was conducted to isolate and select lactic acid bacteria against Colletotrichum sp. causing anthracnose on postharvest capsicum fruits in Binh Tan district, Vinh Long province. As the results, 7 strains of lactic acid bacteria were isolated from traditional fermented products such as kimchi, sauerkraut, and sour shrimp. Antagonistic activity of isolated lactic acid bacteria against Colletotrichum sp. was carried out by antagonistic mycelia and spore methods. The results showed that 7/7 strains of lactic acid bacteria performed the antifungal activity Colletotrichum sp., including 6/7 strains with the well resistance and 1/7 strains with moderate resistance. KC2R strain was noted with the significantly highest antibacterial activity to Colletotrichum sp., with an antagonistic efficiency by the mycelium method of 25.40%, and average inhibition zone by spore method is 5.62 cm. Based on the results of morphological characteristics, the 16S rRNA gene sequencing showed that the KC2R strain were classified belonging to the species of Lactobacillus plantarum with 100% similarity. Research results showed that KC2R strain has the high application potential to post-harvest chili storage.Bệnh thán thư do nấm Colletotrichum sp. xuất hiện ngày càng phổ biến, gây nhiều thiệt hại đến năng suất và chất lượng của nhiều loại cây trồng. Do đó, nghiên cứu này được thực hiện nhằm phân lập và tuyển chọn vi khuẩn lactic có hoạt tính đối kháng nấm Colletotrichum sp. gây bệnh thán thư trên trái ớt sừng sau thu hoạch ở huyện Bình Tân, tỉnh Vĩnh Long. Kết quả nghiên cứu đã phân lập được 7 chủng vi khuẩn lactic từ các sản phẩm lên men truyền thống là kim chi, dưa cải và tôm chua. Hoạt tính đối kháng của các chủng vi khuẩn lactic phân lập được đối với nấm Colletotrichum sp. được thực hiện bằng phương pháp đối kháng sợi nấm và phương pháp đối kháng bào tử. Kết quả cho thấy 7/7 chủng vi khuẩn lactic có hoạt tính đối kháng với nấm Colletotrichum sp., trong đó có 6/7 chủng đối kháng mạnh và 1/7 chủng đối kháng trung bình. Kết quả nghiên cứu chọn được chủng vi khuẩn lactic KC2R có hoạt tính đối kháng mạnh nhất với nấm Colletotrichum sp., với hiệu suất đối kháng bằng phương pháp sợi nấm là 25,40% và trung bình đường kính vòng vô nấm bằng phương pháp bào tử là 5,62 cm. Dựa vào kết quả quan sát đặc điểm hình thái, giải trình tự đoạn gen 16S rRNA cho thấy, chủng vi khuẩn KC2R tương đồng 100% với loài Lactobacillus plantarum. Kết quả nghiên cứu cho thấy, chủng vi khuẩn KC2R có tiềm năng ứng dụng cao trong việc bảo quản ớt sừng sau thu hoạch.

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