ĐÁNH GIÁ TÁC ĐỘNG GIẢM ĐAU, KHÁNG VIÊM CỦA CAO PHÂN ĐOẠN ETHYL ACETAT TỪ CÂY TÍA TÔ DẠI

0 Người đánh giá. Xếp hạng trung bình 0

Tác giả: Sơn Nam Bùi, Thị Phương Liên Hoàng

Ngôn ngữ: vie

Ký hiệu phân loại:

Thông tin xuất bản: Tạp chí Khoa học và Công nghệ - Đại học Thái Nguyên, 2023

Mô tả vật lý: tr.377-384

Bộ sưu tập: Báo, Tạp chí

ID: 339699

Thêm vào giỏ Liên kết toàn văn

Nghiên cứu được thực hiện nhằm đánh giá độc tính cấp, tác động giảm đau và kháng viêm của phân đoạn ethyl acetat từ cây Tía tô dại. Toàn cây trên mặt đất của Tía tô dại được ngấm kiệt với cồn 70%, sau đó cao chiết được lắc phân bố thành các cao phân đoạn n-hexan, chloroform, ethyl acetat và nước. Đánh giá độc tính cấp thực hiện theo hướng dẫn của OECD 423. Thử nghiệm được tiến hành trên 3 chuột đực, 3 chuột cái chủng ICR, liều khởi đầu là 5.000 mg/kg. Kết quả cho thấy không có chuột tử vong hay dấu hiệu bất thường sau 2 tuần theo dõi. Đánh giá tác động giảm đau thông qua mô hình gây đau quặn bằng acid acetic. Đánh giá tác động kháng viêm thông qua mô hình gây viêm bàn chân chuột bằng carrageenan. Ở cả 2 mô hình, chuột được điều trị dự phòng bằng cao thử liều 250 mg/kg, 500 mg/kg, diclofenac 5 mg/kg trong 7 ngày. Ở mô hình giảm đau, cao thử liều 250 mg/kg và 500 mg/kg làm giảm tương ứng 36,78%, 45,53% số lần đau so với lô tá dược. Ở mô hình kháng viêm, cao thử ở cả hai liều cũng thể hiện tác động giảm độ phù chân chuột. Cao phân đoạn ethyl acetat từ cây Tía tô dại không gây độc tính cấp, thể hiện tác động giảm đau, kháng viêm trên mô hình thực nghiệm.This study aims to determine the acute toxicity, analgesic and anti-inflammatory effects of the ethyl acetate fraction from the ethanolic extract of Hyptis suaveolens (L.) Poit. Hyptis suaveolens (L.) Poit was extracted in absolute ethanol 70%. Further, the crude extract was partitioned in n-hexane, chloroform, ethyl acetate, aqueous. The acute toxicity experiment was done as per OECD guidelines 423. The study was done on 3 males and 3 females ICR mice which were administered an initial dose of 5000 mg/kg body weight. No mortality and signs of toxicity were observed in two weeks. Analgesic activity was determined using an acetic acid-induced writhing test on adult male mice. Carrageenan-induced paw oedema model was used for anti-inflammatory activity assessment. In both models, mice were pre-treated for 7 days with the fraction at dose 250 mg/kg, 500 mg/kg and diclofenac 5 mg/kg. In the acetic acid-induced writhing model, the fraction exhibited analgesic effect with 36.78%, 45.53% reduction in writhing response at dose of 250 mg /kg and 500 mg/kg body weight compared to the vehicle group. The anti-inflammatory experiment showed that the extract at both doses significantly (p <
0.05) reduced the paw oedema. The ethyl acetat fraction from Hyptis suaveolens is safe and exhibits analgesic and anti-inflammatory activity.

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