KHẢO SÁT HOẠT TÍNH KHÁNG VI SINH VẬT IN VITRO CỦA PHÂN ĐOẠN CAO KHAI (COPTOSAPELTA FLAVESCENS KORTH. RUBIACEAE)

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Tác giả: Nhựt Trường Nguyễn, Thị Bạch Tuyết Nguyễn

Ngôn ngữ: vie

Ký hiệu phân loại:

Thông tin xuất bản: Tạp chí Khoa học và Công nghệ - Đại học Thái Nguyên, 2023

Mô tả vật lý: tr.385-392

Bộ sưu tập: Metadata

ID: 339700

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Coptosapelta flavescens Korth. Rubiaceae (CF) was reported as a potential herbal with various biological activities, especially antimicrobial activity. To provide more scientific information, this research was conducted to evaluate the antimicrobial activity in vitro of this herbal. CF’s crude extract was collected in Ninh Thuan province in March 2021. Fraction extracts were prepared by partitioning method with solvents of increasing polarity, including n-hexane, chloroform, dichloromethane, ethyl acetate, and methanol. Their antimicrobial activities were determined by agar disc diffusion and agar dilution method. The n- hexane and methanol extracts showed no inhibitory effect on all tested microorganisms. The chloroform and dichloromethane extracts exhibited weak antibacterial activities, against tested Gram-positive bacteria. It was interesting when the ethyl acetate extract resisted all tested microorganisms. In particular, in tested microorganisms, the ethyl acetate extract inhibited the growth of S. aureus ATCC 25923 strongest with MIC of 0.512 mg/mL and diameter of inhibition zone of 22.67 mm. Because S. aureus is a popular agent that causes skin and soft tissue infections, the extract will be a potential active ingredient for developing some preparations for the treatment of tissue infection.Dây khai (Coptosapelta flavescens Korth. Rubiaceae) là một dược liệu quý hiếm, giàu tiềm năng với các tác dụng sinh học khác nhau, trong đó có khả năng kháng vi sinh vật. Với mục đích cung cấp thêm cơ sở khoa học, nghiên cứu thực hiện khảo sát hoạt tính kháng vi sinh vật in vitro của các phân đoạn Cao khai Coptosapelta flavescens Korth. (Rubiaceae family). Sử dụng Cao khai thu thập tại tỉnh Ninh Thuận vào tháng 03/2021 để chiết xuất các cao phân đoạn với các dung môi có độ phân cực tăng dần, bao gồm n –hexan, chloroform, dichloromethan, ethyl acetat và methanol. Đánh giá hiệu quả kháng vi sinh vật của cao phân đoạn bằng phương pháp khuếch tán đĩa thạch dựa vào đường kính vùng ức chế (mm) và sử dụng phương pháp pha loãng trong môi trường thạch để xác định giá trị nồng độ tối thiểu ức chế vi sinh vật (MIC) của cao tiềm năng. Kết quả thu được cho thấy, cao n hexan và cao methnol không thể hiện hoạt tính kháng vi sinh vật. Cao chloroform và cao dichloromethan thể hiện hoạt tính kháng khuẩn yếu, chủ yếu tác động trên chủng vi khuẩn Gram dương. Cao ethyl acetat có khả năng kháng tốt trên 6 chủng vi sinh vật thử nghiệm. Đặc biệt, cao ethyl acetat kháng tốt trên vi khuẩn S. aureus ATCC 25923 – tác nhân thường được phân lập trong các ổ nhiễm trùng da, mô mềm với giá trị MIC đạt 0,512 mg/mL và đường kính vùng ức chế là 22,67 mm. Kết quả nghiên cứu tạo tiền đề để phát triển các dạng bào chế dùng ngoài để rửa vết thương nhiễm trùng.

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